Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AW03_RS12540 Genome accession   NZ_CP007173
Coordinates   2442859..2443476 (-) Length   205 a.a.
NCBI ID   WP_015384132.1    Uniprot ID   -
Organism   Bacillus subtilis HJ5     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2437859..2448476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW03_RS12515 (AW03_024130) rpsT 2438161..2438427 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  AW03_RS12520 (AW03_024150) holA 2438442..2439485 (-) 1044 WP_015384130.1 DNA polymerase III subunit delta -
  AW03_RS21700 - 2439525..2439674 (-) 150 WP_003229985.1 hypothetical protein -
  AW03_RS21235 (AW03_024160) yqzM 2439715..2439849 (+) 135 WP_003229983.1 YqzM family protein -
  AW03_RS12530 (AW03_024170) comEC 2439889..2442219 (-) 2331 WP_041517945.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AW03_RS12535 (AW03_024180) comEB 2442223..2442792 (-) 570 WP_003229978.1 ComE operon protein 2 -
  AW03_RS12540 (AW03_024190) comEA 2442859..2443476 (-) 618 WP_015384132.1 competence protein ComEA Machinery gene
  AW03_RS12545 (AW03_024200) comER 2443560..2444381 (+) 822 WP_004398597.1 late competence protein ComER -
  AW03_RS12550 (AW03_024210) yqeM 2444447..2445190 (-) 744 WP_015384134.1 class I SAM-dependent methyltransferase -
  AW03_RS12555 (AW03_024220) rsfS 2445187..2445543 (-) 357 WP_014480315.1 ribosome silencing factor -
  AW03_RS12560 (AW03_024230) yqeK 2445561..2446121 (-) 561 WP_015483448.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AW03_RS12565 (AW03_024240) nadD 2446111..2446680 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  AW03_RS12570 (AW03_024250) yhbY 2446692..2446982 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  AW03_RS12575 (AW03_024260) aroE 2446976..2447818 (-) 843 WP_015384136.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21744.44 Da        Isoelectric Point: 4.7155

>NTDB_id=117058 AW03_RS12540 WP_015384132.1 2442859..2443476(-) (comEA) [Bacillus subtilis HJ5]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKDLVKQAIPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=117058 AW03_RS12540 WP_015384132.1 2442859..2443476(-) (comEA) [Bacillus subtilis HJ5]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGACCTGGTGAAGCAAGCTATACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCCTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus N315

33.784

100

0.366

Multiple sequence alignment