Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACLV7W_RS10670 Genome accession   NZ_OZ217346
Coordinates   2188198..2189496 (-) Length   432 a.a.
NCBI ID   WP_004255320.1    Uniprot ID   A0A1E9GFM5
Organism   Streptococcus mitis isolate S. mitis D22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2183198..2194496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7W_RS10650 (SMID22_21890) rplI 2184353..2184805 (-) 453 WP_060627336.1 50S ribosomal protein L9 -
  ACLV7W_RS10655 (SMID22_21900) - 2184802..2186775 (-) 1974 WP_000715142.1 DHH family phosphoesterase -
  ACLV7W_RS10660 (SMID22_21910) hpf 2186911..2187459 (-) 549 WP_004255322.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLV7W_RS10665 (SMID22_21920) comFC/cflB 2187539..2188201 (-) 663 WP_004255321.1 ComF family protein Machinery gene
  ACLV7W_RS10670 (SMID22_21930) comFA/cflA 2188198..2189496 (-) 1299 WP_004255320.1 DEAD/DEAH box helicase Machinery gene
  ACLV7W_RS10675 (SMID22_21940) - 2189553..2190188 (+) 636 WP_411865918.1 YigZ family protein -
  ACLV7W_RS10680 - 2190282..2190377 (+) 96 Protein_2062 PH domain-containing protein -
  ACLV7W_RS10685 (SMID22_21950) cysK 2190474..2191400 (+) 927 WP_000029880.1 cysteine synthase A -
  ACLV7W_RS10690 (SMID22_21970) tsf 2191522..2192562 (-) 1041 WP_049499106.1 translation elongation factor Ts -
  ACLV7W_RS10695 (SMID22_21980) rpsB 2192640..2193419 (-) 780 WP_000268459.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49637.59 Da        Isoelectric Point: 8.6690

>NTDB_id=1170480 ACLV7W_RS10670 WP_004255320.1 2188198..2189496(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis D22]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGTYYCRECLLMKRVRSDQSLYYFPQ
EDFPKQDVLKWRGQLTPFQDKVSQGLLQAVDKQEPSLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQKDFACEIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKSRLSPKLKSYIEKQRKTGYPLLIFASEIKKGEQLKEI
IQEQFPNEKIGFVSSITENRLEQVQAFRDRELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1170480 ACLV7W_RS10670 WP_004255320.1 2188198..2189496(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis D22]
ATGAAAGTAAATCCAAATTATCTCGGTCGCTTGTTTACTGAGAATGAATTAACAGAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTACTTACTATTGTAGGGAGTGTTTACTGATGAAGCGAGTCAGGAGTGATCAATCTTTATACTATTTCCCACAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGCGTGGTCAATTAACCCCTTTTCAAGATAAGGTATCACAGGGACTGCT
TCAAGCGGTAGACAAGCAAGAGCCAAGCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TAGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGACGTTTGTTTGGAGCTGTATAAGCGC
CTGCAAAAGGATTTTGCTTGTGAGATAGCCCTCCTACATGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTACTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAATCCTATGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGCTTGAGAATCTTTTTAACAGCAACTTCTACC
GATGAGTTAGATAGGAAGGTTCGCATCGGAGAATTAAAACGATTGAGTTTGCCAAGACGATTTCATGGAAATCCTTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCACTGTTTAGAGAAAAGTCGGTTGTCACCAAAGCTAAAGTCTTATA
TTGAGAAGCAGAGAAAGACAGGTTATCCGTTACTCATTTTTGCTTCAGAGATTAAGAAAGGGGAACAGTTAAAAGAAATC
ATACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTATCACAGAAAATCGATTAGAGCAGGTACAAGCTTT
TCGAGATAGAGAACTGACAATACTTATCAGTACGACGATATTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTGGAAGCCAACCATCGTCTGTTTACCAAGTCTAGTTTAATACAGATTGGAGGACGTGTCGGTCGGAGTATGGACAGA
CCGACAGGAGATTTGCTTTTCTTCCATGACGGGTTAAATGCTTCAATCAAAAAGGCAATTAAGGAAATTCAGCAGATGAA
TAAGGAGGCGGGGCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(292-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E9GFM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae Rx1

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae D39

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae R6

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae TIGR4

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

93.519

100

0.935
  comFA Lactococcus lactis subsp. cremoris KW2

50.117

99.306

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.161

100

0.384

Multiple sequence alignment