Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACLV7D_RS10125 Genome accession   NZ_OZ217345
Coordinates   2128627..2129925 (-) Length   432 a.a.
NCBI ID   WP_218764582.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis F22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2123627..2134925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7D_RS10105 (SMIF22_20050) - 2124681..2125526 (-) 846 WP_050242833.1 3-hydroxybutyryl-CoA dehydrogenase -
  ACLV7D_RS10110 (SMIF22_20060) - 2125608..2126816 (-) 1209 WP_050242834.1 acyl-CoA dehydrogenase family protein -
  ACLV7D_RS10115 (SMIF22_20070) hpf 2127340..2127888 (-) 549 WP_049513313.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLV7D_RS10120 (SMIF22_20080) comFC/cflB 2127968..2128630 (-) 663 WP_218764583.1 ComF family protein Machinery gene
  ACLV7D_RS10125 (SMIF22_20090) comFA/cflA 2128627..2129925 (-) 1299 WP_218764582.1 DEAD/DEAH box helicase Machinery gene
  ACLV7D_RS10130 (SMIF22_20100) - 2129981..2130616 (+) 636 WP_218764581.1 YigZ family protein -
  ACLV7D_RS10135 - 2130710..2130805 (+) 96 Protein_1955 PH domain-containing protein -
  ACLV7D_RS10140 (SMIF22_20110) cysK 2130902..2131828 (+) 927 WP_218764580.1 cysteine synthase A -
  ACLV7D_RS10145 (SMIF22_20130) tsf 2131950..2132990 (-) 1041 WP_000808091.1 translation elongation factor Ts -
  ACLV7D_RS10150 (SMIF22_20140) rpsB 2133070..2133849 (-) 780 WP_014017378.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49681.78 Da        Isoelectric Point: 8.8001

>NTDB_id=1170402 ACLV7D_RS10125 WP_218764582.1 2128627..2129925(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis F22]
MKVNPNYLGRLLTENELTEEERQLAEKLPAMRKEKGKIFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSNQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKHEPTLVHAVTGAGKTEMIYQVVAKVINRGGSVCLASPRIDVCLELYKR
LQKDFSCEIALLHGESKPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
DELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKKCLSPKLKSYIDKQRKTSYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSVTEDRLEKVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHHLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNDSIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1170402 ACLV7D_RS10125 WP_218764582.1 2128627..2129925(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis F22]
ATGAAAGTAAATCCAAATTATCTCGGTCGTTTGCTTACGGAGAATGAATTAACAGAAGAGGAGCGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAAAAGGGGAAGATTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGGAGTAATCAAGCTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCACGAGCCAACCTTAGTACATGCGGTAACAGGGGCTGGAAAGACAGAAATGATTTATCAAGTTG
TGGCTAAGGTGATTAATAGGGGTGGTTCAGTATGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTATACAAGCGC
CTGCAAAAGGATTTTTCTTGCGAGATAGCCCTCCTACATGGAGAATCAAAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAATTTTATCAAGCTTTTGATTTACTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAACCCCATGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGGCTGAGAATCTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGATAAGAAGTGTTTGTCGCCAAAGTTAAAGTCCTATA
TTGATAAGCAGAGAAAGACATCTTATCCGTTACTTATTTTTGCATCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGATTTGTATCTTCTGTAACAGAGGATCGATTAGAGAAAGTACAAGCTTT
TCGAGATGGGGAGCTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACATTCCCTTGTGTGGACGTTTTCG
TAGTAGAGGCCAATCATCATTTGTTTACCAAGTCTAGTTTGATTCAAATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTACTTTTCTTCCATGATGGGTTAAATGATTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

94.676

100

0.947
  comFA/cflA Streptococcus pneumoniae D39

94.676

100

0.947
  comFA/cflA Streptococcus pneumoniae R6

94.676

100

0.947
  comFA/cflA Streptococcus pneumoniae TIGR4

94.444

100

0.944
  comFA/cflA Streptococcus mitis NCTC 12261

92.824

100

0.928
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

50.583

99.306

0.502
  comFA Latilactobacillus sakei subsp. sakei 23K

37.3

100

0.377

Multiple sequence alignment