Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACLV7A_RS09775 Genome accession   NZ_OZ217344
Coordinates   2052508..2053806 (-) Length   432 a.a.
NCBI ID   WP_411864188.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis E22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2047508..2058806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7A_RS09755 (SMIE22_19530) - 2048561..2049406 (-) 846 WP_050242833.1 3-hydroxybutyryl-CoA dehydrogenase -
  ACLV7A_RS09760 (SMIE22_19540) - 2049488..2050696 (-) 1209 WP_050242834.1 acyl-CoA dehydrogenase family protein -
  ACLV7A_RS09765 (SMIE22_19550) hpf 2051221..2051769 (-) 549 WP_050073478.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLV7A_RS09770 (SMIE22_19560) comFC/cflB 2051849..2052511 (-) 663 WP_411864187.1 ComF family protein Machinery gene
  ACLV7A_RS09775 (SMIE22_19570) comFA/cflA 2052508..2053806 (-) 1299 WP_411864188.1 DEAD/DEAH box helicase Machinery gene
  ACLV7A_RS09780 (SMIE22_19580) - 2053863..2054498 (+) 636 WP_411864189.1 YigZ family protein -
  ACLV7A_RS09785 - 2054592..2054687 (+) 96 Protein_1892 PH domain-containing protein -
  ACLV7A_RS09790 (SMIE22_19590) cysK 2054784..2055710 (+) 927 WP_411864190.1 cysteine synthase A -
  ACLV7A_RS09795 (SMIE22_19610) tsf 2055832..2056872 (-) 1041 WP_000808091.1 translation elongation factor Ts -
  ACLV7A_RS09800 (SMIE22_19620) rpsB 2056951..2057730 (-) 780 WP_000268470.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49597.50 Da        Isoelectric Point: 8.5982

>NTDB_id=1170320 ACLV7A_RS09775 WP_411864188.1 2052508..2053806(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis E22]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKGKLVCQRCDSAILDEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVINRGGAVCLASPRIDVCLELYKR
LQNDFSCEIALLHGESKPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
DELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIDKQRKTAYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSVTENRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNDSIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1170320 ACLV7A_RS09775 WP_411864188.1 2052508..2053806(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis E22]
ATGAAAGTAAATCCAAATTATCTTGGTCGCTTGTTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTGTCTGTCAACGTTGTGATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGGAGTGACCAAGCTTTATACTATTTTCCGCAG
GAGGATTTTCCTAAACAGGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAAAAGGTGTCAGAGGGATTGAT
TCAAGCAGTAGACAAGCAAGAGCCAACCTTGGTACATGCGGTGACAGGGGCTGGAAAGACAGAAATGATTTATCAAGTTG
TGGCTAAGGTGATTAATAGGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGATGTTTGTTTGGAGCTGTATAAGCGC
CTGCAAAATGATTTTTCTTGCGAGATAGCCCTCCTACATGGAGAATCAAAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAATTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCTACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTCCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGATAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGATAAGCAGAGAAAGACAGCTTATCCTTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAAAATCGATTAGAGCAGGTTCAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACAACAATCTTGGAGCGTGGAGTTACCTTTCCTTGTGTGGATGTTTTTG
TAGTAGAAGCCAACCATCGTCTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGGGATTTGCTTTTCTTCCATGACGGGTTAAATGATTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae D39

95.37

100

0.954
  comFA/cflA Streptococcus pneumoniae R6

95.37

100

0.954
  comFA/cflA Streptococcus pneumoniae Rx1

95.37

100

0.954
  comFA/cflA Streptococcus pneumoniae TIGR4

95.139

100

0.951
  comFA/cflA Streptococcus mitis SK321

93.519

100

0.935
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA Lactococcus lactis subsp. cremoris KW2

50.35

99.306

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

37.931

100

0.382

Multiple sequence alignment