Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACLV7Z_RS10535 Genome accession   NZ_OZ217343
Coordinates   2152755..2154053 (-) Length   432 a.a.
NCBI ID   WP_218774413.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis G22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2147755..2159053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Z_RS10515 (SMIG22_20810) - 2148808..2149653 (-) 846 WP_050242833.1 3-hydroxybutyryl-CoA dehydrogenase -
  ACLV7Z_RS10520 (SMIG22_20820) - 2149735..2150943 (-) 1209 WP_218774411.1 acyl-CoA dehydrogenase family protein -
  ACLV7Z_RS10525 (SMIG22_20830) hpf 2151468..2152016 (-) 549 WP_050073478.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLV7Z_RS10530 (SMIG22_20840) comFC/cflB 2152096..2152758 (-) 663 WP_173278778.1 ComF family protein Machinery gene
  ACLV7Z_RS10535 (SMIG22_20850) comFA/cflA 2152755..2154053 (-) 1299 WP_218774413.1 DEAD/DEAH box helicase Machinery gene
  ACLV7Z_RS10540 (SMIG22_20860) - 2154109..2154744 (+) 636 WP_112444668.1 YigZ family protein -
  ACLV7Z_RS10545 - 2154838..2154933 (+) 96 Protein_2035 PH domain-containing protein -
  ACLV7Z_RS10550 (SMIG22_20870) cysK 2155030..2155956 (+) 927 WP_175880407.1 cysteine synthase A -
  ACLV7Z_RS10555 (SMIG22_20890) tsf 2156078..2157118 (-) 1041 WP_000808091.1 translation elongation factor Ts -
  ACLV7Z_RS10560 (SMIG22_20900) rpsB 2157197..2157976 (-) 780 WP_000268466.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49552.38 Da        Isoelectric Point: 7.3091

>NTDB_id=1170241 ACLV7Z_RS10535 WP_218774413.1 2152755..2154053(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis G22]
MKVNLDYLGRLFTENELTEEECQLAEKLPAMRKEKGKLFCQRCDSAILEEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQNVLKWRGQLTPFQEKVSEGLIQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQDDFACEIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
DELDRKVRLGELKRLSLPRRFHGNPLIIPKPVWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNDSIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1170241 ACLV7Z_RS10535 WP_218774413.1 2152755..2154053(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis G22]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTGTTTACTGAGAATGAATTAACAGAAGAGGAATGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAGCTTTTCTGTCAACGTTGTGATAGTGCTATTCTGGAGGAATGGTATCTAC
CTATTGGTGCCTACTATTGTCGGGAATGCTTGCTGATGAAGAGAGTTAGGAGTGATCAAGCTTTATACTATTTTCCACAG
GAGGATTTTCCTAAACAGAATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAAAAGGTGTCAGAGGGACTGAT
TCAAGCAGTAGACAAGCAGGAGCCAACTTTAGTTCATGCGGTGACAGGGGCTGGAAAGACAGAAATGATTTATCAAGTTG
TGGCTAAGGTGATCAATGCGGGAGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGATGTTTGTTTGGAGCTTTATAAGCGT
TTGCAAGATGATTTTGCTTGTGAGATAGCCCTCCTACATGGAGAATCAGAACCGTACTTCCGAACCCCATTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCATTTGATTTACTGATAGTGGATGAAGTAGATGCATTTCCTTATGTTG
ACAATCCAACGCTTTACCATGCTGTCAAGAACAGTGTAAAGGAGAATGGGCTGAGAATCTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAGAAAGGTCCGTTTAGGAGAATTGAAACGATTGAGTTTGCCTAGACGATTTCATGGAAATCCGTTGAT
TATTCCTAAGCCAGTTTGGTTATCGGATTTTAATCGCTATTTGGATAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAAAAGCAGAGAAAGACAGCTTATCCTTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAGGTGCAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTCTGTTTACCAAGTCAAGCTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGATTAAATGATTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

96.065

100

0.961
  comFA/cflA Streptococcus pneumoniae D39

96.065

100

0.961
  comFA/cflA Streptococcus pneumoniae R6

96.065

100

0.961
  comFA/cflA Streptococcus pneumoniae TIGR4

95.833

100

0.958
  comFA/cflA Streptococcus mitis SK321

94.444

100

0.944
  comFA/cflA Streptococcus mitis NCTC 12261

93.981

100

0.94
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.673

100

0.391

Multiple sequence alignment