Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACLV65_RS10120 Genome accession   NZ_OZ217342
Coordinates   2065374..2066672 (-) Length   432 a.a.
NCBI ID   WP_411865180.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis B22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2060374..2071672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV65_RS10100 (SMIB22_19930) - 2061425..2062270 (-) 846 WP_411865177.1 3-hydroxybutyryl-CoA dehydrogenase -
  ACLV65_RS10105 (SMIB22_19940) - 2062352..2063560 (-) 1209 WP_050242834.1 acyl-CoA dehydrogenase family protein -
  ACLV65_RS10110 (SMIB22_19950) hpf 2064087..2064635 (-) 549 WP_411865178.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACLV65_RS10115 (SMIB22_19960) comFC/cflB 2064715..2065377 (-) 663 WP_411865179.1 ComF family protein Machinery gene
  ACLV65_RS10120 (SMIB22_19970) comFA/cflA 2065374..2066672 (-) 1299 WP_411865180.1 DEAD/DEAH box helicase Machinery gene
  ACLV65_RS10125 (SMIB22_19980) - 2066729..2067364 (+) 636 WP_411865181.1 YigZ family protein -
  ACLV65_RS10130 (SMIB22_19990) cysK 2067650..2068576 (+) 927 WP_000029881.1 cysteine synthase A -
  ACLV65_RS10135 (SMIB22_20010) tsf 2068698..2069738 (-) 1041 WP_173234333.1 translation elongation factor Ts -
  ACLV65_RS10140 (SMIB22_20020) rpsB 2069818..2070597 (-) 780 WP_000268466.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49612.51 Da        Isoelectric Point: 8.4375

>NTDB_id=1170155 ACLV65_RS10120 WP_411865180.1 2065374..2066672(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis B22]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKGKLVCQRCDSAILDEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLIQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVINRGGAVCLASPRIDVCLELYKR
LQNDFSCEIALLHGESKPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
DELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIDKQRKTAYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSITEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNDSIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1170155 ACLV65_RS10120 WP_411865180.1 2065374..2066672(-) (comFA/cflA) [Streptococcus mitis isolate S. mitis B22]
ATGAAAGTAAATCCAAACTATCTCGGTCGTTTGTTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTGTCTGTCAACGTTGTGATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGGAGTGACCAAGCTTTATACTATTTTCCGCAG
GAGGATTTTCCTAAACAGGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAAAAGGTGTCAGAGGGATTGAT
TCAAGCAGTAGACAAGCAAGAGCCAACCTTGGTACATGCGGTGACAGGGGCTGGAAAGACAGAAATGATTTATCAAGTTG
TGGCTAAGGTGATTAATAGGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGATGTTTGTTTGGAGCTGTATAAGCGC
CTGCAAAATGATTTTTCTTGCGAGATAGCCCTCCTACATGGAGAATCAAAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAATTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCTACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
GATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTCCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGATAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGATAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGATTTGTCTCTTCTATCACAGAAGATCGATTAGAGCAGGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGGGTTACCTTCCCTTGTGTGGACGTTTTCG
TAGTAGAGGCTAATCATCGTCTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGATTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

95.37

100

0.954
  comFA/cflA Streptococcus pneumoniae D39

95.37

100

0.954
  comFA/cflA Streptococcus pneumoniae R6

95.37

100

0.954
  comFA/cflA Streptococcus pneumoniae TIGR4

95.139

100

0.951
  comFA/cflA Streptococcus mitis SK321

93.519

100

0.935
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA Lactococcus lactis subsp. cremoris KW2

50.35

99.306

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

38.161

100

0.384

Multiple sequence alignment