Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACH8EK_RS10280 Genome accession   NZ_OZ197910
Coordinates   1956677..1957537 (+) Length   286 a.a.
NCBI ID   WP_003689814.1    Uniprot ID   A0AA44U9R8
Organism   Neisseria gonorrhoeae isolate SGC-24-001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1951677..1962537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH8EK_RS10265 dapD 1952202..1953023 (-) 822 WP_003689804.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  ACH8EK_RS10270 pgi 1953233..1954861 (-) 1629 WP_395372569.1 glucose-6-phosphate isomerase -
  ACH8EK_RS10275 pilG 1955371..1956603 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  ACH8EK_RS10280 pilD 1956677..1957537 (+) 861 WP_003689814.1 A24 family peptidase Machinery gene
  ACH8EK_RS10285 coaE 1957534..1958166 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  ACH8EK_RS10290 - 1958159..1958368 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  ACH8EK_RS10295 pilF 1958454..1960130 (-) 1677 WP_003689817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACH8EK_RS10305 - 1960468..1960914 (-) 447 WP_003689818.1 DUF441 domain-containing protein -
  ACH8EK_RS10310 - 1960918..1961892 (-) 975 WP_003689819.1 polyprenyl synthetase family protein -
  ACH8EK_RS10315 rplU 1962116..1962424 (+) 309 WP_003691838.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31332.34 Da        Isoelectric Point: 8.9561

>NTDB_id=1169734 ACH8EK_RS10280 WP_003689814.1 1956677..1957537(+) (pilD) [Neisseria gonorrhoeae isolate SGC-24-001]
MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVR

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1169734 ACH8EK_RS10280 WP_003689814.1 1956677..1957537(+) (pilD) [Neisseria gonorrhoeae isolate SGC-24-001]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCAGTTTTGGGGCTGCTGGTCGGCAGCTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTTATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGACGATGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGTCCCAAATGCCGTGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATCTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATAGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCTCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCGGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGAGATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(134-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

100

100

1
  pilD Vibrio cholerae strain A1552

44.792

100

0.451
  pilD Vibrio campbellii strain DS40M4

45.652

96.503

0.441
  pilD Acinetobacter baumannii D1279779

47.727

92.308

0.441
  pilD Acinetobacter nosocomialis M2

47.727

92.308

0.441

Multiple sequence alignment