Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACFCTL_RS07495 Genome accession   NZ_OZ182546
Coordinates   1634333..1635196 (+) Length   287 a.a.
NCBI ID   WP_014841651.1    Uniprot ID   I7HJU7
Organism   Legionella pneumophila isolate ZIB8567     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629333..1640196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCTL_RS07475 - 1629827..1630153 (-) 327 WP_011213802.1 DUF1820 family protein -
  ACFCTL_RS07480 - 1630336..1631079 (-) 744 WP_014841649.1 methyltransferase domain-containing protein -
  ACFCTL_RS07485 pilB 1631338..1633062 (+) 1725 WP_010947251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFCTL_RS07490 pilC 1633068..1634288 (+) 1221 WP_014841650.1 type II secretion system F family protein Machinery gene
  ACFCTL_RS07495 pilD 1634333..1635196 (+) 864 WP_014841651.1 A24 family peptidase Machinery gene
  ACFCTL_RS07500 - 1635210..1636415 (-) 1206 WP_014841652.1 cyclic nucleotide-binding domain-containing protein -
  ACFCTL_RS07505 - 1636523..1637116 (-) 594 WP_011213808.1 hypothetical protein -
  ACFCTL_RS07510 - 1637132..1637587 (-) 456 WP_014841654.1 hypothetical protein -
  ACFCTL_RS07515 - 1637721..1638536 (+) 816 WP_014841655.1 pyruvate, water dikinase regulatory protein -
  ACFCTL_RS07520 - 1638656..1640104 (-) 1449 WP_014841656.1 bifunctional 2-methylcitrate dehydratase/aconitate hydratase -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 32756.36 Da        Isoelectric Point: 7.8442

>NTDB_id=1167984 ACFCTL_RS07495 WP_014841651.1 1634333..1635196(+) (pilD) [Legionella pneumophila isolate ZIB8567]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIITWYIGYWM

Nucleotide


Download         Length: 864 bp        

>NTDB_id=1167984 ACFCTL_RS07495 WP_014841651.1 1634333..1635196(+) (pilD) [Legionella pneumophila isolate ZIB8567]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCCGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTGCCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGATAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGACGTTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGTTATTCATTAATCTGTTTTATCTGATGACAGGTAAGGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACGGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATTATCACTTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-125)

Type IV leader peptidase family (Peptidase_A24)

(136-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7HJU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

49.11

97.909

0.481
  pilD Acinetobacter nosocomialis M2

47.985

95.122

0.456
  pilD Acinetobacter baumannii D1279779

47.273

95.819

0.453
  pilD Vibrio campbellii strain DS40M4

44.964

96.864

0.436
  pilD Neisseria gonorrhoeae MS11

42.446

96.864

0.411

Multiple sequence alignment