Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   AW02_RS12350 Genome accession   NZ_CP007165
Coordinates   2611629..2612066 (-) Length   145 a.a.
NCBI ID   WP_003153088.1    Uniprot ID   -
Organism   Bacillus velezensis NJN-6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2606629..2617066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW02_RS12300 (AW02_024480) sinI 2607014..2607187 (+) 174 WP_003153105.1 anti-repressor SinI Regulator
  AW02_RS12305 (AW02_024490) sinR 2607221..2607556 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  AW02_RS12310 (AW02_024500) tasA 2607604..2608389 (-) 786 WP_003153102.1 biofilm matrix protein TasA -
  AW02_RS12315 (AW02_024510) sipW 2608453..2609037 (-) 585 WP_003153100.1 signal peptidase I SipW -
  AW02_RS12320 (AW02_024520) tapA 2609009..2609680 (-) 672 WP_046341384.1 amyloid fiber anchoring/assembly protein TapA -
  AW02_RS12325 (AW02_024530) - 2609939..2610268 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  AW02_RS12330 (AW02_024540) - 2610308..2610487 (-) 180 WP_003153093.1 YqzE family protein -
  AW02_RS12335 (AW02_024550) comGG 2610544..2610921 (-) 378 WP_003153092.1 competence type IV pilus minor pilin ComGG Machinery gene
  AW02_RS12340 (AW02_024560) comGF 2610922..2611317 (-) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  AW02_RS12345 (AW02_024570) comGE 2611331..2611645 (-) 315 WP_046341385.1 competence type IV pilus minor pilin ComGE -
  AW02_RS12350 (AW02_024580) comGD 2611629..2612066 (-) 438 WP_003153088.1 competence type IV pilus minor pilin ComGD Machinery gene
  AW02_RS12355 (AW02_024590) comGC 2612056..2612322 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  AW02_RS12360 (AW02_024600) comGB 2612369..2613406 (-) 1038 WP_003153086.1 competence type IV pilus assembly protein ComGB Machinery gene
  AW02_RS12365 (AW02_024610) comGA 2613393..2614463 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  AW02_RS12370 (AW02_024620) - 2614655..2615605 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  AW02_RS12375 (AW02_024630) - 2615751..2617052 (+) 1302 WP_014305416.1 hemolysin family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16324.85 Da        Isoelectric Point: 10.3725

>NTDB_id=116716 AW02_RS12350 WP_003153088.1 2611629..2612066(-) (comGD) [Bacillus velezensis NJN-6]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPREHKYK
LQSAGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIRLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=116716 AW02_RS12350 WP_003153088.1 2611629..2612066(-) (comGD) [Bacillus velezensis NJN-6]
TTGAACAATAACAGGCGGACAGAAAACGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTTCAAAAAGATA
TTCAGCTTGCGCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAGAGAGCATAAATACAAA
CTGCAGTCAGCCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCGATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

54.795

100

0.552

Multiple sequence alignment