Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AACH22_RS17265 Genome accession   NZ_OY970400
Coordinates   3627513..3628589 (-) Length   358 a.a.
NCBI ID   WP_072273816.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans isolate BFRIG     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3622513..3633589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH22_RS17225 (BFRIG_03485) - 3623405..3624202 (+) 798 WP_048688306.1 YqhG family protein -
  AACH22_RS17230 - 3624247..3624432 (-) 186 WP_048688303.1 YqzE family protein -
  AACH22_RS17235 (BFRIG_03486) comGG 3624525..3624911 (-) 387 WP_048688300.1 competence type IV pilus minor pilin ComGG -
  AACH22_RS17240 (BFRIG_03487) comGF 3624904..3625383 (-) 480 WP_081088666.1 competence type IV pilus minor pilin ComGF -
  AACH22_RS17245 (BFRIG_03488) - 3625331..3625669 (-) 339 WP_048688295.1 hypothetical protein -
  AACH22_RS17250 (BFRIG_03489) comGD 3625656..3626099 (-) 444 WP_338565956.1 competence type IV pilus minor pilin ComGD -
  AACH22_RS17255 (BFRIG_03490) comGC 3626107..3626418 (-) 312 WP_048688337.1 competence type IV pilus major pilin ComGC -
  AACH22_RS17260 (BFRIG_03491) comGB 3626495..3627529 (-) 1035 WP_202186366.1 competence type IV pilus assembly protein ComGB -
  AACH22_RS17265 (BFRIG_03492) comGA 3627513..3628589 (-) 1077 WP_072273816.1 competence type IV pilus ATPase ComGA Machinery gene
  AACH22_RS17270 (BFRIG_03493) - 3628694..3629074 (-) 381 WP_048682187.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AACH22_RS17275 (BFRIG_03494) - 3629290..3629991 (+) 702 WP_048682184.1 helix-turn-helix domain-containing protein -
  AACH22_RS17280 (BFRIG_03495) - 3630077..3630319 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  AACH22_RS17285 (BFRIG_03496) - 3630372..3631472 (-) 1101 WP_072273817.1 SAM-dependent methyltransferase -
  AACH22_RS17290 (BFRIG_03497) - 3631781..3632407 (-) 627 WP_048682180.1 MBL fold metallo-hydrolase -
  AACH22_RS17295 (BFRIG_03498) - 3632641..3632814 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39774.44 Da        Isoelectric Point: 8.4720

>NTDB_id=1162230 AACH22_RS17265 WP_072273816.1 3627513..3628589(-) (comGA) [Peribacillus frigoritolerans isolate BFRIG]
MISIEKTAEKILTRAIVESASDIHIFFRGEGPLIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLPCKGDCALACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GKATVSYRQLKDEIGKAVAMGYVDAQEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=1162230 AACH22_RS17265 WP_072273816.1 3627513..3628589(-) (comGA) [Peribacillus frigoritolerans isolate BFRIG]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAGTCGGCATCGGATATCCACATTTTTTT
TCGCGGAGAGGGGCCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTATCATTTTTTGAAGCTG
AAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGCGCCATCACC
ATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACATTATATTCCCTGCTTCACCAC
GCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCCGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCAGTCCGTGCCGCATTAACCGGTCATTTGATACTAACGACCATGCAT
ACGAGGGATGCCCAGGGCGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGACTTTGATTGG
CGTGACAGCGCAGCGGCTGGTTGAATTACGATGTCTTCCGTGTAAAGGGGACTGTGCTTTAGCTTGCAAAATGACTGCGA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCGGTTGCGATGGGTTATGTGGATGCTCAGGA
ATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

38.506

97.207

0.374
  pilB Haemophilus influenzae Rd KW20

37.356

97.207

0.363

Multiple sequence alignment