Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BH81_RS01880 Genome accession   NZ_CP007039
Coordinates   399584..400648 (+) Length   354 a.a.
NCBI ID   WP_025258132.1    Uniprot ID   -
Organism   Pseudomonas cichorii JBC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394584..405648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BH81_RS01865 (PCH70_03800) - 394865..395719 (+) 855 WP_025258129.1 sulfurtransferase -
  BH81_RS01870 (PCH70_03810) - 395771..396817 (-) 1047 WP_025258130.1 hydrolase -
  BH81_RS01875 (PCH70_03820) - 396934..399375 (-) 2442 WP_025258131.1 penicillin-binding protein 1A -
  BH81_RS01880 (PCH70_03830) pilM 399584..400648 (+) 1065 WP_025258132.1 pilus assembly protein PilM Machinery gene
  BH81_RS01885 (PCH70_03840) - 400648..401220 (+) 573 WP_025258133.1 PilN domain-containing protein -
  BH81_RS01890 (PCH70_03850) pilO 401217..401840 (+) 624 WP_025258134.1 type 4a pilus biogenesis protein PilO -
  BH81_RS01895 (PCH70_03860) - 401837..402364 (+) 528 WP_025258135.1 pilus assembly protein PilP -
  BH81_RS01900 (PCH70_03870) pilQ 402412..404511 (+) 2100 WP_025258136.1 type IV pilus secretin PilQ Machinery gene
  BH81_RS01905 (PCH70_03880) aroK 404516..405034 (+) 519 WP_025258137.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38127.62 Da        Isoelectric Point: 4.6928

>NTDB_id=116187 BH81_RS01880 WP_025258132.1 399584..400648(+) (pilM) [Pseudomonas cichorii JBC1]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNAKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLSAQLGNDHDQLTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLSDDYVSEVLNPFKDALVQQVSRSLQFFFAAGQYNTVDYIMLAGGTASIPGLEHLIQKRLGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=116187 BH81_RS01880 WP_025258132.1 399584..400648(+) (pilM) [Pseudomonas cichorii JBC1]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCTACCTCGGTAAAACTCCTTGAGTT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTATGCGGTCGAACCGCTGCCGGCCAATGCCGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGACAGGCGTTGTCGCGCGTGCTGGTCAAGGCCAAGACGAACGCCAAAATAGTGGCGGTT
GCTGTGGCGGGCTCGGCGGTCATCACCAAGACCATCGAGATGGACGCCGGTCTGTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATTCCCTATCCCCTCGAAGAAGTCGCCATCGATTTTGAAGTCCAGGGCTATTCGGCTC
GCAATCCCGAGCGCGTCGAAGTGCTGCTGGCCGCCTGCCGCAAGGAAAACGTGGAAGTACGTGAGGCCGCTTTGGCGCTG
GCCGGGCTGACGGCCCGTGTCGTCGACGTGGAGGCCTATGCACTGGAGCGTTCTTTCGGTCTGCTGTCTGCCCAACTGGG
CAATGACCACGATCAACTGACCGTTGCCGTGGTGGACATCGGCGCAACCATGACCACCCTGAGCGTGCTGCATCACGGGC
GCATCATCTATACCCGCGAGCAATTGTTCGGTGGGCGCCAGCTGACCGAGGAAATTCAGCGTCGCTATGGCCTGTCCATG
GAAGAAGCGGGGCTTGCGAAGAAGCAGGGCGGGCTGTCCGACGATTACGTCAGTGAAGTGTTGAACCCCTTCAAGGATGC
TCTGGTGCAGCAAGTGTCTCGCTCGCTGCAGTTTTTCTTTGCTGCCGGGCAGTACAACACCGTCGACTACATCATGCTGG
CGGGCGGTACGGCTTCCATTCCGGGACTTGAGCACTTGATCCAGAAACGTCTTGGCACGCCAACCCTGGTCGCGAACCCT
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAACGCCGGTGCATTGGCCAGTGATGCTCCCGCGTTGATGATCGCCTGCGG
TCTTGCCCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.367

100

0.554
  comM Acinetobacter nosocomialis M2

55.367

100

0.554
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment