Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   QOS64_RS13845 Genome accession   NZ_OX460973
Coordinates   2792491..2793105 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2787491..2798105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOS64_RS13815 rpsT 2787794..2788060 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  QOS64_RS13820 holA 2788077..2789118 (-) 1042 Protein_2681 DNA polymerase III subunit delta -
  QOS64_RS13825 - 2789158..2789309 (-) 152 Protein_2682 hypothetical protein -
  QOS64_RS13830 - 2789350..2789484 (+) 135 WP_003152870.1 YqzM family protein -
  QOS64_RS13835 comEC 2789503..2791854 (-) 2352 WP_168985061.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  QOS64_RS13840 - 2791855..2792424 (-) 570 WP_003152868.1 ComE operon protein 2 -
  QOS64_RS13845 comEA 2792491..2793105 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  QOS64_RS13850 comER 2793164..2793985 (+) 822 WP_168985062.1 late competence protein ComER -
  QOS64_RS13855 - 2794054..2794791 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  QOS64_RS13860 rsfS 2794788..2795144 (-) 357 WP_007408260.1 ribosome silencing factor -
  QOS64_RS13865 yqeK 2795162..2795722 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  QOS64_RS13870 - 2795712..2796281 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  QOS64_RS13875 yhbY 2796292..2796582 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  QOS64_RS13880 aroE 2796576..2797418 (-) 843 WP_168985063.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=1159460 QOS64_RS13845 WP_015240245.1 2792491..2793105(-) (comEA) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1159460 QOS64_RS13845 WP_015240245.1 2792491..2793105(-) (comEA) [Bacillus velezensis strain SAF3325 substr. 0 isolate SAF3325]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment