Detailed information NTG1158469

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QEN43_RS13150
• Genome accession: NZ_OX458333
• Coordinates: 3046393..3047613 (-)
• Length: 406 a.a.
• NCBI ID: WP_036269683.1
• Uniprot ID: -
• Organism: Methylocaldum szegediense isolate Msz(Nor)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3041393..3052613

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEN43_RS13125 (MSZNOR_3152)	-	3042382..3042858 (-)	477	WP_235726648.1	Uma2 family endonuclease	-
QEN43_RS13130 (MSZNOR_3153)	metK	3042893..3044062 (-)	1170	WP_026611087.1	methionine adenosyltransferase	-
QEN43_RS13135 (MSZNOR_3154)	-	3044181..3044666 (-)	486	WP_026611086.1	hypothetical protein	-
QEN43_RS13140 (MSZNOR_3155)	ppa	3044842..3045390 (+)	549	WP_026611085.1	inorganic diphosphatase	-
QEN43_RS13145 (MSZNOR_3156)	-	3045432..3046274 (+)	843	WP_026611084.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
QEN43_RS13150 (MSZNOR_3158)	pilU	3046393..3047613 (-)	1221	WP_036269683.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QEN43_RS13155 (MSZNOR_3159)	-	3048057..3048293 (+)	237	WP_036269109.1	hypothetical protein	-
QEN43_RS13160 (MSZNOR_3160)	-	3048473..3052606 (-)	4134	WP_317963287.1	chemotaxis protein CheB	-

Sequence

Protein

• Length: 406 a.a.
• Molecular weight: 45259.90 Da
• Isoelectric Point: 6.1953

>NTDB_id=1158469 QEN43_RS13150 WP_036269683.1 3046393..3047613(-) (pilU) [Methylocaldum szegediense isolate Msz(Nor)]
MKFKNYLKLLVYKDGSDLYLTAGAPPAAKFQGKLMPIDKVALTDEMIKEIAYSIMDESQRQDFERRPEMNLAISEEGIGR
FRVNIFKQRNMHAMVVRNIKVNIPKVEDLGLPDVLKKVIMENRGLILFVGATGSGKSTSLAALIDYRNSHSAGHIITIED
PIEFIHPHKRCLVNQREVGVDTQSYEDALKNTLRQAPDVILIGEIRSQDTMEYAINFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLMDLSLNLRAFVSQRLVPTVDGKRTAAIEILLGTPLVCDLIRKGEVHAIKEVMEKSEGLGMQTFDSHL
VKLYQAGRITAEEAIRNADSANNVRLKISLNQPLASKKASAKSTVLLQDQTNEKTESPQTAESPLDTMDLSLEPVADESE
VAGEGH

Nucleotide

• Length: 1221 bp

>NTDB_id=1158469 QEN43_RS13150 WP_036269683.1 3046393..3047613(-) (pilU) [Methylocaldum szegediense isolate Msz(Nor)]
ATGAAATTCAAGAACTACCTCAAACTCCTGGTCTACAAGGATGGTTCCGATCTTTATCTCACCGCTGGCGCCCCGCCCGC
CGCCAAGTTTCAGGGGAAGCTGATGCCGATCGATAAAGTCGCGTTAACGGACGAGATGATCAAAGAGATCGCCTATTCGA
TTATGGACGAGAGCCAGCGCCAGGATTTTGAACGGCGCCCAGAGATGAACCTCGCCATCAGCGAGGAAGGAATTGGCCGT
TTTCGCGTGAACATCTTTAAGCAGCGCAACATGCATGCAATGGTCGTGCGGAATATCAAGGTCAACATTCCTAAGGTAGA
GGATCTGGGTCTGCCGGACGTCCTCAAAAAGGTCATCATGGAAAACCGGGGGCTGATTCTGTTCGTAGGCGCCACCGGCT
CCGGCAAATCCACCTCACTGGCGGCGCTGATCGACTACCGCAACAGCCATTCCGCCGGACATATCATCACCATCGAAGAC
CCGATCGAGTTCATTCACCCGCACAAAAGATGCCTAGTTAATCAGCGGGAGGTAGGCGTCGATACCCAGAGTTACGAGGA
TGCTCTTAAGAACACGCTTCGGCAGGCACCGGATGTCATCCTGATCGGCGAGATCCGTTCACAGGACACGATGGAATACG
CCATCAATTTCGCAGAGACAGGCCACTTGTGCCTATCGACCCTACACGCCAACAACGCGAATCAGGCGCTCGACCGAATC
ATTAACTTCTTCCCCGAAGAACGGCGAAACCAGCTATTAATGGACTTGTCCCTAAACTTGCGGGCTTTCGTCTCCCAACG
CCTGGTGCCCACGGTCGACGGCAAACGCACGGCCGCCATCGAGATTCTGCTCGGAACACCCCTAGTCTGCGATCTAATCC
GCAAGGGCGAAGTCCACGCAATCAAGGAAGTCATGGAGAAGTCCGAAGGCTTAGGAATGCAGACTTTCGATAGCCATTTG
GTGAAGCTCTATCAGGCCGGCCGGATCACCGCTGAAGAAGCCATCCGCAACGCGGATTCGGCCAACAACGTCCGATTGAA
AATCAGCCTGAACCAGCCGCTTGCTTCCAAAAAAGCATCCGCGAAGAGTACGGTGCTTCTTCAAGATCAGACCAACGAAA
AAACCGAGAGCCCCCAAACCGCCGAGTCGCCGTTGGACACGATGGATCTGAGCCTGGAGCCCGTCGCCGACGAAAGCGAA
GTAGCCGGGGAGGGTCATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (40-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.963	86.453	0.544
pilU	Acinetobacter baylyi ADP1	54.67	89.655	0.49
pilU	Vibrio cholerae strain A1552	50.716	85.961	0.436