Detailed information NTG1158081

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP56_RS14200
• Genome accession: NZ_OX419652
• Coordinates: 2643169..2643786 (-)
• Length: 205 a.a.
• NCBI ID: WP_032726193.1
• Uniprot ID: -
• Organism: Bacillus subtilis isolate NRS6131
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2638169..2648786

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP56_RS14170 (NRS6131_14130)	rpsT	2638471..2638737 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP56_RS14175 (NRS6131_14135)	holA	2638752..2639795 (-)	1044	WP_021480061.1	DNA polymerase III subunit delta	-
KJP56_RS14180	-	2639835..2639984 (-)	150	WP_003229985.1	hypothetical protein	-
KJP56_RS14185 (NRS6131_14140)	yqzM	2640025..2640159 (+)	135	WP_003229983.1	YqzM family protein	-
KJP56_RS14190 (NRS6131_14145)	comEC	2640199..2642529 (-)	2331	WP_213419120.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP56_RS14195 (NRS6131_14150)	comEB	2642533..2643102 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP56_RS14200 (NRS6131_14155)	comEA	2643169..2643786 (-)	618	WP_032726193.1	competence protein ComEA	Machinery gene
KJP56_RS14205 (NRS6131_14160)	comER	2643870..2644691 (+)	822	WP_032726195.1	late competence protein ComER	-
KJP56_RS14210 (NRS6131_14165)	yqeM	2644757..2645500 (-)	744	WP_213419121.1	class I SAM-dependent methyltransferase	-
KJP56_RS14215 (NRS6131_14170)	rsfS	2645497..2645853 (-)	357	WP_014480315.1	ribosome silencing factor	-
KJP56_RS14220 (NRS6131_14175)	yqeK	2645871..2646431 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP56_RS14225 (NRS6131_14180)	nadD	2646421..2646990 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP56_RS14230 (NRS6131_14185)	yhbY	2647002..2647292 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP56_RS14235 (NRS6131_14190)	aroE	2647286..2648128 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21753.50 Da
• Isoelectric Point: 4.8417

>NTDB_id=1158081 KJP56_RS14200 WP_032726193.1 2643169..2643786(-) (comEA) [Bacillus subtilis isolate NRS6131]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1158081 KJP56_RS14200 WP_032726193.1 2643169..2643786(-) (comEA) [Bacillus subtilis isolate NRS6131]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376