Detailed information NTG1158000

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP60_RS14225
• Genome accession: NZ_OX419580
• Coordinates: 2689039..2689656 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6116
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2684039..2694656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP60_RS14195 (NRS6116_14235)	rpsT	2684341..2684607 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP60_RS14200 (NRS6116_14240)	holA	2684622..2685665 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP60_RS14205	-	2685705..2685854 (-)	150	WP_003229985.1	hypothetical protein	-
KJP60_RS14210 (NRS6116_14245)	yqzM	2685895..2686029 (+)	135	WP_003229983.1	YqzM family protein	-
KJP60_RS14215 (NRS6116_14250)	comEC	2686069..2688399 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP60_RS14220 (NRS6116_14255)	comEB	2688403..2688972 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP60_RS14225 (NRS6116_14260)	comEA	2689039..2689656 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP60_RS14230 (NRS6116_14265)	comER	2689740..2690561 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP60_RS14235 (NRS6116_14270)	yqeM	2690627..2691370 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP60_RS14240 (NRS6116_14275)	rsfS	2691367..2691723 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP60_RS14245 (NRS6116_14280)	yqeK	2691741..2692301 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP60_RS14250 (NRS6116_14285)	nadD	2692291..2692860 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP60_RS14255 (NRS6116_14290)	yhbY	2692872..2693162 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP60_RS14260 (NRS6116_14295)	aroE	2693156..2693998 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1158000 KJP60_RS14225 WP_004398514.1 2689039..2689656(-) (comEA) [Bacillus subtilis isolate NRS6116]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1158000 KJP60_RS14225 WP_004398514.1 2689039..2689656(-) (comEA) [Bacillus subtilis isolate NRS6116]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39