Detailed information NTG1157838

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP45_RS12715
• Genome accession: NZ_OX419578
• Coordinates: 2475090..2475707 (-)
• Length: 205 a.a.
• NCBI ID: WP_122894878.1
• Uniprot ID: -
• Organism: Bacillus subtilis isolate NRS6128
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2470090..2480707

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP45_RS12685 (NRS6128_12600)	rpsT	2470392..2470658 (+)	267	WP_131227637.1	30S ribosomal protein S20	-
KJP45_RS12690 (NRS6128_12605)	holA	2470673..2471716 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
KJP45_RS12695	-	2471756..2471905 (-)	150	WP_003229985.1	hypothetical protein	-
KJP45_RS12700 (NRS6128_12610)	yqzM	2471946..2472080 (+)	135	WP_003229983.1	YqzM family protein	-
KJP45_RS12705 (NRS6128_12615)	comEC	2472120..2474450 (-)	2331	WP_213414606.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP45_RS12710 (NRS6128_12620)	comEB	2474454..2475023 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP45_RS12715 (NRS6128_12625)	comEA	2475090..2475707 (-)	618	WP_122894878.1	competence protein ComEA	Machinery gene
KJP45_RS12720 (NRS6128_12630)	comER	2475791..2476612 (+)	822	WP_213414605.1	late competence protein ComER	-
KJP45_RS12725 (NRS6128_12635)	yqeM	2476678..2477421 (-)	744	WP_015384134.1	class I SAM-dependent methyltransferase	-
KJP45_RS12730 (NRS6128_12640)	rsfS	2477418..2477774 (-)	357	WP_014477391.1	ribosome silencing factor	-
KJP45_RS12735 (NRS6128_12645)	yqeK	2477792..2478352 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP45_RS12740 (NRS6128_12650)	nadD	2478342..2478911 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP45_RS12745 (NRS6128_12655)	yhbY	2478923..2479213 (-)	291	WP_213414604.1	ribosome assembly RNA-binding protein YhbY	-
KJP45_RS12750 (NRS6128_12660)	aroE	2479207..2480049 (-)	843	WP_076458133.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21827.58 Da
• Isoelectric Point: 4.9552

>NTDB_id=1157838 KJP45_RS12715 WP_122894878.1 2475090..2475707(-) (comEA) [Bacillus subtilis isolate NRS6128]
MNWLNQHKKTIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSKQADEAQVNLAEVLQDGTVVYIPKKGEETAVQQGTGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157838 KJP45_RS12715 WP_122894878.1 2475090..2475707(-) (comEA) [Bacillus subtilis isolate NRS6128]
ATGAATTGGTTGAATCAGCATAAGAAAACAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAGTAAACAAGCAGACGAAGCGCAAGTAAATTTGGCCGA
GGTTCTTCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTACCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGGCCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.073	100	0.971
comEA	Staphylococcus aureus MW2	36.364	100	0.39
comEA	Staphylococcus aureus N315	36.364	100	0.39