Detailed information NTG1157684

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP51_RS12740
• Genome accession: NZ_OX419576
• Coordinates: 2486542..2487159 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6105
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2481542..2492159

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP51_RS12710 (NRS6105_12630)	rpsT	2481843..2482109 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP51_RS12715 (NRS6105_12635)	holA	2482124..2483167 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
KJP51_RS12720	-	2483207..2483356 (-)	150	WP_003229985.1	hypothetical protein	-
KJP51_RS12725 (NRS6105_12640)	yqzM	2483397..2483531 (+)	135	WP_003229983.1	YqzM family protein	-
KJP51_RS12730 (NRS6105_12645)	comEC	2483572..2485902 (-)	2331	WP_069704087.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP51_RS12735 (NRS6105_12650)	comEB	2485906..2486475 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP51_RS12740 (NRS6105_12655)	comEA	2486542..2487159 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP51_RS12745 (NRS6105_12660)	comER	2487243..2488064 (+)	822	WP_014480313.1	late competence protein ComER	-
KJP51_RS12750 (NRS6105_12665)	yqeM	2488130..2488873 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP51_RS12755 (NRS6105_12670)	rsfS	2488870..2489226 (-)	357	WP_014480315.1	ribosome silencing factor	-
KJP51_RS12760 (NRS6105_12675)	yqeK	2489244..2489804 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP51_RS12765 (NRS6105_12680)	nadD	2489794..2490363 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP51_RS12770 (NRS6105_12685)	yhbY	2490375..2490665 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP51_RS12775 (NRS6105_12690)	aroE	2490659..2491501 (-)	843	WP_041351695.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1157684 KJP51_RS12740 WP_004398514.1 2486542..2487159(-) (comEA) [Bacillus subtilis isolate NRS6105]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157684 KJP51_RS12740 WP_004398514.1 2486542..2487159(-) (comEA) [Bacillus subtilis isolate NRS6105]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39