Detailed information NTG1157513

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP57_RS14220
• Genome accession: NZ_OX419573
• Coordinates: 2692943..2693560 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6085
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2687943..2698560

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP57_RS14190 (NRS6085_14205)	rpsT	2688245..2688511 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP57_RS14195 (NRS6085_14210)	holA	2688526..2689569 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
KJP57_RS14200	-	2689609..2689758 (-)	150	WP_120363468.1	hypothetical protein	-
KJP57_RS14205 (NRS6085_14215)	yqzM	2689799..2689933 (+)	135	WP_003229983.1	YqzM family protein	-
KJP57_RS14210 (NRS6085_14220)	comEC	2689973..2692303 (-)	2331	WP_213407810.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP57_RS14215 (NRS6085_14225)	comEB	2692307..2692876 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP57_RS14220 (NRS6085_14230)	comEA	2692943..2693560 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP57_RS14225 (NRS6085_14235)	comER	2693644..2694465 (+)	822	WP_014480313.1	late competence protein ComER	-
KJP57_RS14230 (NRS6085_14240)	yqeM	2694531..2695274 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP57_RS14235 (NRS6085_14245)	rsfS	2695271..2695627 (-)	357	WP_041333759.1	ribosome silencing factor	-
KJP57_RS14240 (NRS6085_14250)	yqeK	2695645..2696205 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP57_RS14245 (NRS6085_14255)	nadD	2696195..2696764 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP57_RS14250 (NRS6085_14260)	yhbY	2696776..2697066 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP57_RS14255 (NRS6085_14265)	aroE	2697060..2697902 (-)	843	WP_128738025.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1157513 KJP57_RS14220 WP_004398514.1 2692943..2693560(-) (comEA) [Bacillus subtilis isolate NRS6085]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157513 KJP57_RS14220 WP_004398514.1 2692943..2693560(-) (comEA) [Bacillus subtilis isolate NRS6085]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39