Detailed information NTG1157432

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP59_RS13375
• Genome accession: NZ_OX419570
• Coordinates: 2583434..2584051 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6134
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2578434..2589051

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP59_RS13345 (NRS6134_13310)	rpsT	2578736..2579002 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP59_RS13350 (NRS6134_13315)	holA	2579017..2580060 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP59_RS13355	-	2580100..2580249 (-)	150	WP_003229985.1	hypothetical protein	-
KJP59_RS13360 (NRS6134_13320)	yqzM	2580290..2580424 (+)	135	WP_003229983.1	YqzM family protein	-
KJP59_RS13365 (NRS6134_13325)	comEC	2580464..2582794 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP59_RS13370 (NRS6134_13330)	comEB	2582798..2583367 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP59_RS13375 (NRS6134_13335)	comEA	2583434..2584051 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP59_RS13380 (NRS6134_13340)	comER	2584135..2584956 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP59_RS13385 (NRS6134_13345)	yqeM	2585022..2585765 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP59_RS13390 (NRS6134_13350)	rsfS	2585762..2586118 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP59_RS13395 (NRS6134_13355)	yqeK	2586136..2586696 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP59_RS13400 (NRS6134_13360)	nadD	2586686..2587255 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP59_RS13405 (NRS6134_13365)	yhbY	2587267..2587557 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP59_RS13410 (NRS6134_13370)	aroE	2587551..2588393 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1157432 KJP59_RS13375 WP_004398514.1 2583434..2584051(-) (comEA) [Bacillus subtilis isolate NRS6134]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157432 KJP59_RS13375 WP_004398514.1 2583434..2584051(-) (comEA) [Bacillus subtilis isolate NRS6134]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39