Detailed information NTG1157260

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP42_RS12975
• Genome accession: NZ_OX419567
• Coordinates: 2526149..2526766 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6107
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2521149..2531766

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP42_RS12945 (NRS6107_12895)	rpsT	2521451..2521717 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP42_RS12950 (NRS6107_12900)	holA	2521732..2522775 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP42_RS12955	-	2522815..2522964 (-)	150	WP_003229985.1	hypothetical protein	-
KJP42_RS12960 (NRS6107_12905)	yqzM	2523005..2523139 (+)	135	WP_003229983.1	YqzM family protein	-
KJP42_RS12965 (NRS6107_12910)	comEC	2523179..2525509 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP42_RS12970 (NRS6107_12915)	comEB	2525513..2526082 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP42_RS12975 (NRS6107_12920)	comEA	2526149..2526766 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP42_RS12980 (NRS6107_12925)	comER	2526850..2527671 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP42_RS12985 (NRS6107_12930)	yqeM	2527737..2528480 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP42_RS12990 (NRS6107_12935)	rsfS	2528477..2528833 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP42_RS12995 (NRS6107_12940)	yqeK	2528851..2529411 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP42_RS13000 (NRS6107_12945)	nadD	2529401..2529970 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP42_RS13005 (NRS6107_12950)	yhbY	2529982..2530272 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP42_RS13010 (NRS6107_12955)	aroE	2530266..2531108 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1157260 KJP42_RS12975 WP_004398514.1 2526149..2526766(-) (comEA) [Bacillus subtilis isolate NRS6107]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157260 KJP42_RS12975 WP_004398514.1 2526149..2526766(-) (comEA) [Bacillus subtilis isolate NRS6107]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39