Detailed information NTG1157176

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP64_RS12750
• Genome accession: NZ_OX419565
• Coordinates: 2481504..2482121 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6145
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2476504..2487121

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP64_RS12720 (NRS6145_12600)	rpsT	2476805..2477071 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP64_RS12725 (NRS6145_12605)	holA	2477086..2478129 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
KJP64_RS12730	-	2478169..2478318 (-)	150	WP_003229985.1	hypothetical protein	-
KJP64_RS12735 (NRS6145_12610)	yqzM	2478359..2478493 (+)	135	WP_003229983.1	YqzM family protein	-
KJP64_RS12740 (NRS6145_12615)	comEC	2478534..2480864 (-)	2331	WP_069704087.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP64_RS12745 (NRS6145_12620)	comEB	2480868..2481437 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP64_RS12750 (NRS6145_12625)	comEA	2481504..2482121 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP64_RS12755 (NRS6145_12630)	comER	2482205..2483026 (+)	822	WP_014480313.1	late competence protein ComER	-
KJP64_RS12760 (NRS6145_12635)	yqeM	2483092..2483835 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP64_RS12765 (NRS6145_12640)	rsfS	2483832..2484188 (-)	357	WP_014480315.1	ribosome silencing factor	-
KJP64_RS12770 (NRS6145_12645)	yqeK	2484206..2484766 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP64_RS12775 (NRS6145_12650)	nadD	2484756..2485325 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP64_RS12780 (NRS6145_12655)	yhbY	2485337..2485627 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP64_RS12785 (NRS6145_12660)	aroE	2485621..2486463 (-)	843	WP_041351695.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1157176 KJP64_RS12750 WP_004398514.1 2481504..2482121(-) (comEA) [Bacillus subtilis isolate NRS6145]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157176 KJP64_RS12750 WP_004398514.1 2481504..2482121(-) (comEA) [Bacillus subtilis isolate NRS6145]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39