Detailed information NTG1157092

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP48_RS13835
• Genome accession: NZ_OX419564
• Coordinates: 2630811..2631428 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6127
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2625811..2636428

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP48_RS13805 (NRS6127_13735)	rpsT	2626113..2626379 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP48_RS13810 (NRS6127_13740)	holA	2626394..2627437 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP48_RS13815	-	2627477..2627626 (-)	150	WP_003229985.1	hypothetical protein	-
KJP48_RS13820 (NRS6127_13745)	yqzM	2627667..2627801 (+)	135	WP_003229983.1	YqzM family protein	-
KJP48_RS13825 (NRS6127_13750)	comEC	2627841..2630171 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP48_RS13830 (NRS6127_13755)	comEB	2630175..2630744 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP48_RS13835 (NRS6127_13760)	comEA	2630811..2631428 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP48_RS13840 (NRS6127_13765)	comER	2631512..2632333 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP48_RS13845 (NRS6127_13770)	yqeM	2632399..2633142 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP48_RS13850 (NRS6127_13775)	rsfS	2633139..2633495 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP48_RS13855 (NRS6127_13780)	yqeK	2633513..2634073 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP48_RS13860 (NRS6127_13785)	nadD	2634063..2634632 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP48_RS13865 (NRS6127_13790)	yhbY	2634644..2634934 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP48_RS13870 (NRS6127_13795)	aroE	2634928..2635770 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1157092 KJP48_RS13835 WP_004398514.1 2630811..2631428(-) (comEA) [Bacillus subtilis isolate NRS6127]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157092 KJP48_RS13835 WP_004398514.1 2630811..2631428(-) (comEA) [Bacillus subtilis isolate NRS6127]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39