Detailed information NTG1157011

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP66_RS13515
• Genome accession: NZ_OX419563
• Coordinates: 2601428..2602045 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6153
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2596428..2607045

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP66_RS13485 (NRS6153_13415)	rpsT	2596730..2596996 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP66_RS13490 (NRS6153_13420)	holA	2597011..2598054 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP66_RS13495	-	2598094..2598243 (-)	150	WP_003229985.1	hypothetical protein	-
KJP66_RS13500 (NRS6153_13425)	yqzM	2598284..2598418 (+)	135	WP_003229983.1	YqzM family protein	-
KJP66_RS13505 (NRS6153_13430)	comEC	2598458..2600788 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP66_RS13510 (NRS6153_13435)	comEB	2600792..2601361 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP66_RS13515 (NRS6153_13440)	comEA	2601428..2602045 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP66_RS13520 (NRS6153_13445)	comER	2602129..2602950 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP66_RS13525 (NRS6153_13450)	yqeM	2603016..2603759 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP66_RS13530 (NRS6153_13455)	rsfS	2603756..2604112 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP66_RS13535 (NRS6153_13460)	yqeK	2604130..2604690 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP66_RS13540 (NRS6153_13465)	nadD	2604680..2605249 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP66_RS13545 (NRS6153_13470)	yhbY	2605261..2605551 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP66_RS13550 (NRS6153_13475)	aroE	2605545..2606387 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1157011 KJP66_RS13515 WP_004398514.1 2601428..2602045(-) (comEA) [Bacillus subtilis isolate NRS6153]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1157011 KJP66_RS13515 WP_004398514.1 2601428..2602045(-) (comEA) [Bacillus subtilis isolate NRS6153]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39