Detailed information NTG1156848

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP68_RS12905
• Genome accession: NZ_OX419559
• Coordinates: 2502791..2503408 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6099
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2497791..2508408

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP68_RS12875 (NRS6099_12810)	rpsT	2498093..2498359 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP68_RS12880 (NRS6099_12815)	holA	2498374..2499417 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP68_RS12885	-	2499457..2499606 (-)	150	WP_003229985.1	hypothetical protein	-
KJP68_RS12890 (NRS6099_12820)	yqzM	2499647..2499781 (+)	135	WP_003229983.1	YqzM family protein	-
KJP68_RS12895 (NRS6099_12825)	comEC	2499821..2502151 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP68_RS12900 (NRS6099_12830)	comEB	2502155..2502724 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP68_RS12905 (NRS6099_12835)	comEA	2502791..2503408 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP68_RS12910 (NRS6099_12840)	comER	2503492..2504313 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP68_RS12915 (NRS6099_12845)	yqeM	2504379..2505122 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP68_RS12920 (NRS6099_12850)	rsfS	2505119..2505475 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP68_RS12925 (NRS6099_12855)	yqeK	2505493..2506053 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP68_RS12930 (NRS6099_12860)	nadD	2506043..2506612 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP68_RS12935 (NRS6099_12865)	yhbY	2506624..2506914 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP68_RS12940 (NRS6099_12870)	aroE	2506908..2507750 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1156848 KJP68_RS12905 WP_004398514.1 2502791..2503408(-) (comEA) [Bacillus subtilis isolate NRS6099]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1156848 KJP68_RS12905 WP_004398514.1 2502791..2503408(-) (comEA) [Bacillus subtilis isolate NRS6099]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39