Detailed information NTG1156690

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP65_RS13175
• Genome accession: NZ_OX419555
• Coordinates: 2512936..2513553 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6181
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2507936..2518553

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP65_RS13145 (NRS6181_13105)	rpsT	2508245..2508511 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP65_RS13150 (NRS6181_13110)	holA	2508526..2509569 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
KJP65_RS13155	-	2509609..2509758 (-)	150	WP_003229985.1	hypothetical protein	-
KJP65_RS13160 (NRS6181_13115)	yqzM	2509799..2509933 (+)	135	WP_003229983.1	YqzM family protein	-
KJP65_RS13165 (NRS6181_13120)	comEC	2509973..2512303 (-)	2331	WP_074794436.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP65_RS13170 (NRS6181_13125)	comEB	2512300..2512869 (-)	570	WP_015714288.1	ComE operon protein 2	-
KJP65_RS13175 (NRS6181_13130)	comEA	2512936..2513553 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP65_RS13180 (NRS6181_13135)	comER	2513637..2514458 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP65_RS13185 (NRS6181_13140)	yqeM	2514524..2515267 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP65_RS13190 (NRS6181_13145)	rsfS	2515264..2515620 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP65_RS13195 (NRS6181_13150)	yqeK	2515638..2516198 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP65_RS13200 (NRS6181_13155)	nadD	2516188..2516757 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP65_RS13205 (NRS6181_13160)	yhbY	2516769..2517059 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP65_RS13210 (NRS6181_13165)	aroE	2517053..2517895 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1156690 KJP65_RS13175 WP_004398514.1 2512936..2513553(-) (comEA) [Bacillus subtilis isolate NRS6181]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1156690 KJP65_RS13175 WP_004398514.1 2512936..2513553(-) (comEA) [Bacillus subtilis isolate NRS6181]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39