Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   RG32_RS02365 Genome accession   NZ_CP010132
Coordinates   417191..417823 (+) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli strain C10     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 412191..422823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG32_RS02330 (RG32_02210) yheS 412360..414273 (+) 1914 WP_000634798.1 ABC transporter ATP-binding protein -
  RG32_RS02335 (RG32_02215) yheT 414273..415295 (+) 1023 WP_000057405.1 hydrolase -
  RG32_RS02340 (RG32_02220) yheU 415289..415507 (+) 219 WP_000907085.1 YheU family protein -
  RG32_RS02345 (RG32_02225) prkB 415561..416430 (+) 870 WP_001274680.1 phosphoribulokinase -
  RG32_RS02350 (RG32_02230) yhfA 416485..416889 (-) 405 WP_001148904.1 OsmC family protein -
  RG32_RS02365 (RG32_02240) crp 417191..417823 (+) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  RG32_RS02370 (RG32_02245) yhfK 417874..419964 (+) 2091 WP_001295162.1 YccS/YhfK family putative transporter -
  RG32_RS02375 (RG32_02250) argD 420031..421251 (-) 1221 WP_000963797.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  RG32_RS02380 (RG32_02255) pabA 421337..421900 (-) 564 WP_000601847.1 aminodeoxychorismate synthase component 2 -
  RG32_RS02385 (RG32_02260) fic 421932..422534 (-) 603 WP_001280624.1 putative adenosine monophosphate-protein transferase Fic -
  RG32_RS02390 (RG32_02265) yhfG 422524..422691 (-) 168 WP_000736860.1 YhfG family protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=115649 RG32_RS02365 WP_000242755.1 417191..417823(+) (crp) [Escherichia coli strain C10]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=115649 RG32_RS02365 WP_000242755.1 417191..417823(+) (crp) [Escherichia coli strain C10]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAACCTGGCAAAACAACCAGATGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467

Multiple sequence alignment