Detailed information NTG1156449

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP53_RS12995
• Genome accession: NZ_OX419551
• Coordinates: 2529503..2530120 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6190
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2524503..2535120

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP53_RS12965 (NRS6190_12920)	rpsT	2524805..2525071 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP53_RS12970 (NRS6190_12925)	holA	2525086..2526129 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP53_RS12975	-	2526169..2526318 (-)	150	WP_003229985.1	hypothetical protein	-
KJP53_RS12980 (NRS6190_12930)	yqzM	2526359..2526493 (+)	135	WP_003229983.1	YqzM family protein	-
KJP53_RS12985 (NRS6190_12935)	comEC	2526533..2528863 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP53_RS12990 (NRS6190_12940)	comEB	2528867..2529436 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP53_RS12995 (NRS6190_12945)	comEA	2529503..2530120 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP53_RS13000 (NRS6190_12950)	comER	2530204..2531025 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP53_RS13005 (NRS6190_12955)	yqeM	2531091..2531834 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP53_RS13010 (NRS6190_12960)	rsfS	2531831..2532187 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP53_RS13015 (NRS6190_12965)	yqeK	2532205..2532765 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP53_RS13020 (NRS6190_12970)	nadD	2532755..2533324 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP53_RS13025 (NRS6190_12975)	yhbY	2533336..2533626 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP53_RS13030 (NRS6190_12980)	aroE	2533620..2534462 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1156449 KJP53_RS12995 WP_004398514.1 2529503..2530120(-) (comEA) [Bacillus subtilis isolate NRS6190]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1156449 KJP53_RS12995 WP_004398514.1 2529503..2530120(-) (comEA) [Bacillus subtilis isolate NRS6190]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39