Detailed information NTG1156365

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KJP69_RS12910
• Genome accession: NZ_OX419550
• Coordinates: 2502793..2503410 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis isolate NRS6187
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2497793..2508410

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP69_RS12880 (NRS6187_12805)	rpsT	2498095..2498361 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KJP69_RS12885 (NRS6187_12810)	holA	2498376..2499419 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
KJP69_RS12890	-	2499459..2499608 (-)	150	WP_003229985.1	hypothetical protein	-
KJP69_RS12895 (NRS6187_12815)	yqzM	2499649..2499783 (+)	135	WP_003229983.1	YqzM family protein	-
KJP69_RS12900 (NRS6187_12820)	comEC	2499823..2502153 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KJP69_RS12905 (NRS6187_12825)	comEB	2502157..2502726 (-)	570	WP_003229978.1	ComE operon protein 2	-
KJP69_RS12910 (NRS6187_12830)	comEA	2502793..2503410 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
KJP69_RS12915 (NRS6187_12835)	comER	2503494..2504315 (+)	822	WP_004398597.1	late competence protein ComER	-
KJP69_RS12920 (NRS6187_12840)	yqeM	2504381..2505124 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
KJP69_RS12925 (NRS6187_12845)	rsfS	2505121..2505477 (-)	357	WP_003229971.1	ribosome silencing factor	-
KJP69_RS12930 (NRS6187_12850)	yqeK	2505495..2506055 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KJP69_RS12935 (NRS6187_12855)	nadD	2506045..2506614 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KJP69_RS12940 (NRS6187_12860)	yhbY	2506626..2506916 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KJP69_RS12945 (NRS6187_12865)	aroE	2506910..2507752 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=1156365 KJP69_RS12910 WP_004398514.1 2502793..2503410(-) (comEA) [Bacillus subtilis isolate NRS6187]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1156365 KJP69_RS12910 WP_004398514.1 2502793..2503410(-) (comEA) [Bacillus subtilis isolate NRS6187]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39