Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   QML75_RS00070 Genome accession   NZ_OX346408
Coordinates   14627..15145 (+) Length   172 a.a.
NCBI ID   WP_016250467.1    Uniprot ID   A0A0H2QMK6
Organism   Enterococcus cecorum isolate CIRMBP-1302     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 14523..84218 14627..15145 within 0


Gene organization within MGE regions


Location: 14523..84218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QML75_RS00070 (CIRMBP1302_00014) ssb 14627..15145 (+) 519 WP_016250467.1 single-stranded DNA-binding protein Machinery gene
  QML75_RS00075 (CIRMBP1302_00015) rpsR 15175..15411 (+) 237 WP_016250466.1 30S ribosomal protein S18 -
  QML75_RS00080 (CIRMBP1302_00016) - 15600..16013 (+) 414 WP_016250465.1 NUDIX hydrolase -
  QML75_RS00085 (CIRMBP1302_00017) - 16050..16364 (+) 315 WP_235426786.1 NAD(P)H-dependent oxidoreductase -
  QML75_RS00090 (CIRMBP1302_00018) - 16590..20429 (+) 3840 WP_016250463.1 YSIRK signal domain/LPXTG anchor domain surface protein -
  QML75_RS00095 (CIRMBP1302_00019) - 20638..22089 (+) 1452 WP_050047225.1 DHH family phosphoesterase -
  QML75_RS00100 (CIRMBP1302_00020) rplI 22102..22554 (+) 453 WP_016250461.1 50S ribosomal protein L9 -
  QML75_RS00105 (CIRMBP1302_00021) - 22761..23093 (+) 333 WP_018345237.1 hypothetical protein -
  QML75_RS00110 (CIRMBP1302_00022) dnaB 23250..24620 (+) 1371 WP_016250459.1 replicative DNA helicase -
  QML75_RS00115 (CIRMBP1302_00023) - 24670..24990 (-) 321 WP_047241755.1 hypothetical protein -
  QML75_RS00120 (CIRMBP1302_00024) - 25410..27578 (+) 2169 WP_016250457.1 PTS transporter subunit IIBC -
  QML75_RS00125 (CIRMBP1302_00025) - 27679..28476 (+) 798 WP_047241753.1 endonuclease/exonuclease/phosphatase family protein -
  QML75_RS00130 (CIRMBP1302_00026) - 28735..30027 (+) 1293 WP_047241769.1 adenylosuccinate synthase -
  QML75_RS00135 (CIRMBP1302_00027) - 30451..30660 (+) 210 WP_152666054.1 hypothetical protein -
  QML75_RS00140 (CIRMBP1302_00028) - 30913..31320 (+) 408 WP_235426086.1 hypothetical protein -
  QML75_RS00145 (CIRMBP1302_00029) - 31593..32795 (-) 1203 WP_047241751.1 MFS transporter -
  QML75_RS00150 (CIRMBP1302_00030) - 33108..34739 (-) 1632 WP_016250453.1 ATP-binding cassette domain-containing protein -
  QML75_RS00155 (CIRMBP1302_00031) - 35186..35518 (+) 333 WP_016250439.1 PTS sugar transporter subunit IIB -
  QML75_RS00160 (CIRMBP1302_00032) - 35538..35900 (+) 363 WP_016250438.1 PTS lactose/cellobiose transporter subunit IIA -
  QML75_RS00165 (CIRMBP1302_00033) - 35959..37047 (+) 1089 WP_047241768.1 MupG family TIM beta-alpha barrel fold protein -
  QML75_RS00170 (CIRMBP1302_00034) - 37237..38613 (+) 1377 WP_050047223.1 6-phospho-beta-glucosidase -
  QML75_RS00175 - 38753..39040 (+) 288 Protein_34 DUF871 domain-containing protein -
  QML75_RS00180 (CIRMBP1302_00035) - 39311..40747 (+) 1437 WP_047241748.1 6-phospho-beta-glucosidase -
  QML75_RS00185 (CIRMBP1302_00036) - 40812..41306 (+) 495 WP_047241747.1 hypothetical protein -
  QML75_RS00190 (CIRMBP1302_00037) celB 41329..42675 (+) 1347 WP_047241746.1 PTS cellobiose transporter subunit IIC -
  QML75_RS00195 (CIRMBP1302_00038) - 42922..44139 (-) 1218 WP_001291561.1 tyrosine-type recombinase/integrase -
  QML75_RS00200 (CIRMBP1302_00039) - 44221..44424 (-) 204 WP_000814511.1 excisionase -
  QML75_RS00205 - 44408..44659 (+) 252 WP_001845478.1 hypothetical protein -
  QML75_RS00210 (CIRMBP1302_00040) - 44885..45115 (-) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  QML75_RS00215 (CIRMBP1302_00041) - 45112..45534 (-) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  QML75_RS00220 (CIRMBP1302_00042) - 46038..46391 (+) 354 WP_001227347.1 helix-turn-helix transcriptional regulator -
  QML75_RS00225 - 46451..46582 (-) 132 WP_080961458.1 cysteine-rich KTR domain-containing protein -
  QML75_RS00230 (CIRMBP1302_00043) - 46610..47176 (-) 567 WP_047241745.1 GNAT family protein -
  QML75_RS00235 (CIRMBP1302_00044) tet(M) 47349..49268 (-) 1920 WP_047241744.1 tetracycline resistance ribosomal protection protein Tet(M) -
  QML75_RS00240 - 49284..49400 (-) 117 WP_001814923.1 tetracycline resistance determinant leader peptide -
  QML75_RS00245 (CIRMBP1302_00045) - 49645..50577 (-) 933 WP_001224318.1 conjugal transfer protein -
  QML75_RS00250 (CIRMBP1302_00046) - 50574..51575 (-) 1002 WP_000769868.1 bifunctional lysozyme/C40 family peptidase -
  QML75_RS00255 (CIRMBP1302_00047) - 51572..53749 (-) 2178 WP_282007801.1 YtxH domain-containing protein -
  QML75_RS00260 (CIRMBP1302_00048) - 53752..56199 (-) 2448 WP_047241742.1 ATP-binding protein -
  QML75_RS00265 - 56183..56575 (-) 393 WP_000723888.1 conjugal transfer protein -
  QML75_RS00270 (CIRMBP1302_00049) - 56664..57161 (-) 498 WP_000342539.1 antirestriction protein ArdA -
  QML75_RS00275 (CIRMBP1302_00050) - 57278..57499 (-) 222 WP_001009056.1 hypothetical protein -
  QML75_RS00280 (CIRMBP1302_00051) mobT 57542..58747 (-) 1206 WP_000398284.1 MobT family relaxase -
  QML75_RS00285 - 58770..58922 (-) 153 WP_000879507.1 hypothetical protein -
  QML75_RS00290 (CIRMBP1302_00052) - 58925..60310 (-) 1386 WP_000813488.1 FtsK/SpoIIIE domain-containing protein -
  QML75_RS00295 (CIRMBP1302_00053) - 60339..60725 (-) 387 WP_000985015.1 YdcP family protein -
  QML75_RS00300 (CIRMBP1302_00054) - 60741..61055 (-) 315 WP_000420682.1 YdcP family protein -
  QML75_RS00305 (CIRMBP1302_00055) - 61433..62146 (-) 714 WP_047241741.1 GntR family transcriptional regulator -
  QML75_RS00310 (CIRMBP1302_00056) - 62326..63807 (+) 1482 WP_047241740.1 family 1 glycosylhydrolase -
  QML75_RS00315 (CIRMBP1302_00057) - 63854..65101 (-) 1248 WP_047241739.1 glutamate-5-semialdehyde dehydrogenase -
  QML75_RS00320 (CIRMBP1302_00058) proB 65117..65920 (-) 804 WP_235426847.1 glutamate 5-kinase -
  QML75_RS11830 - 66054..66221 (-) 168 WP_326929994.1 hypothetical protein -
  QML75_RS11835 (CIRMBP1302_00059) - 66236..66505 (-) 270 WP_326929993.1 hypothetical protein -
  QML75_RS11840 (CIRMBP1302_00060) - 66456..66746 (-) 291 WP_326929961.1 hypothetical protein -
  QML75_RS00330 (CIRMBP1302_00061) - 66969..67490 (+) 522 WP_047241737.1 hypothetical protein -
  QML75_RS00335 (CIRMBP1302_00062) radA 67503..68873 (+) 1371 WP_047241736.1 DNA repair protein RadA Machinery gene
  QML75_RS00340 (CIRMBP1302_00063) - 69073..70179 (+) 1107 WP_016250426.1 PIN/TRAM domain-containing protein -
  QML75_RS00345 (CIRMBP1302_00064) rfbC 70219..70776 (+) 558 WP_047241735.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  QML75_RS00350 (CIRMBP1302_00065) - 70992..71297 (+) 306 WP_047241734.1 hypothetical protein -
  QML75_RS00355 (CIRMBP1302_00066) - 71466..71702 (+) 237 WP_235426088.1 GNAT family N-acetyltransferase -
  QML75_RS00360 (CIRMBP1302_00067) gltX 71938..73395 (+) 1458 WP_047241733.1 glutamate--tRNA ligase -
  QML75_RS00365 (CIRMBP1302_00068) epsC 73820..74353 (+) 534 WP_016250421.1 serine O-acetyltransferase EpsC -
  QML75_RS00370 (CIRMBP1302_00069) cysS 74346..75752 (+) 1407 WP_047241732.1 cysteine--tRNA ligase -
  QML75_RS00375 (CIRMBP1302_00070) - 75752..76144 (+) 393 WP_016250419.1 Mini-ribonuclease 3 -
  QML75_RS00380 (CIRMBP1302_00071) rlmB 76141..76944 (+) 804 WP_047241731.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QML75_RS00385 (CIRMBP1302_00072) - 76948..77484 (+) 537 WP_047241730.1 NYN domain-containing protein -
  QML75_RS00390 - 77665..77880 (+) 216 Protein_79 sigma factor -
  QML75_RS00395 - 77884..78066 (+) 183 Protein_80 transposase -
  QML75_RS00400 (CIRMBP1302_00075) tnpB 78083..79225 (+) 1143 WP_047241729.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
  QML75_RS00405 - 79521..79688 (+) 168 WP_160297721.1 hypothetical protein -
  QML75_RS00410 (CIRMBP1302_00076) pgmB 79733..80383 (-) 651 WP_047241728.1 beta-phosphoglucomutase -
  QML75_RS00415 (CIRMBP1302_00077) - 80534..80743 (+) 210 WP_047241727.1 hypothetical protein -
  QML75_RS00420 (CIRMBP1302_00078) thiT 81007..81585 (+) 579 WP_047241726.1 energy-coupled thiamine transporter ThiT -
  QML75_RS00425 (CIRMBP1302_00079) - 81654..82610 (-) 957 WP_050047220.1 biotin--[acetyl-CoA-carboxylase] ligase -

Sequence


Protein


Download         Length: 172 a.a.        Molecular weight: 19042.89 Da        Isoelectric Point: 4.7093

>NTDB_id=1155187 QML75_RS00070 WP_016250467.1 14627..15145(+) (ssb) [Enterococcus cecorum isolate CIRMBP-1302]
MINNVVLVGRLTRDPDLRYTSSGVAVATFSLAVNRNFTSQNGERETDFINCVIWRKPAETLANYARKGTLIGLTGRIQTR
NYENQQGQRVYVTEVVADNFQLLESKAVNDQRRQAAGNFDNNVSQPFNNNNNSFDQPASSQPFSGMPGFDRDASNTPLGG
SSIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=1155187 QML75_RS00070 WP_016250467.1 14627..15145(+) (ssb) [Enterococcus cecorum isolate CIRMBP-1302]
TTGATTAATAATGTTGTATTAGTAGGTAGATTAACAAGAGACCCTGATTTACGTTACACTTCTTCTGGTGTTGCGGTGGC
TACTTTTAGTTTAGCTGTGAACCGTAATTTTACCAGCCAAAATGGAGAAAGAGAAACGGACTTTATCAATTGTGTCATTT
GGCGTAAACCAGCTGAAACCTTAGCAAATTACGCTAGAAAAGGGACATTAATTGGTTTGACCGGACGTATTCAAACGAGA
AATTATGAAAACCAACAAGGTCAACGTGTATACGTAACTGAAGTGGTTGCCGATAATTTCCAATTATTAGAGTCTAAAGC
AGTCAACGATCAACGTCGTCAAGCTGCCGGAAACTTTGATAATAATGTCTCACAACCATTTAACAATAATAACAATAGCT
TTGATCAGCCAGCTTCATCTCAACCATTTAGTGGAATGCCTGGCTTTGACCGTGATGCAAGTAACACACCACTTGGCGGA
TCAAGCATTGACATTTCAGATGATGATTTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0H2QMK6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

60.345

100

0.61

  ssbA Bacillus subtilis subsp. subtilis str. 168

53.409

100

0.547

  ssbB Bacillus subtilis subsp. subtilis str. 168

59.434

61.628

0.366


Multiple sequence alignment