Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DXE63_RS09710 Genome accession   NZ_LT992458
Coordinates   1835619..1836116 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus isolate 7_4623     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1830619..1841116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE63_RS09670 - 1830663..1831649 (+) 987 WP_000161315.1 ROK family glucokinase -
  DXE63_RS09675 - 1831649..1831978 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DXE63_RS09680 - 1831975..1832598 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DXE63_RS09685 comGA 1832650..1833624 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DXE63_RS09690 comGB 1833596..1834666 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  DXE63_RS09695 comGC 1834680..1834991 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DXE63_RS09700 comGD 1834969..1835415 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  DXE63_RS09705 comGE 1835402..1835701 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  DXE63_RS09710 comGF 1835619..1836116 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  DXE63_RS09715 - 1836213..1836359 (+) 147 WP_001789879.1 hypothetical protein -
  DXE63_RS09720 - 1836349..1836873 (+) 525 WP_001015120.1 shikimate kinase -
  DXE63_RS09725 gcvT 1837032..1838123 (+) 1092 WP_114621347.1 glycine cleavage system aminomethyltransferase GcvT -
  DXE63_RS09730 gcvPA 1838143..1839489 (+) 1347 WP_031795510.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DXE63_RS09735 gcvPB 1839482..1840954 (+) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1149338 DXE63_RS09710 WP_001825340.1 1835619..1836116(+) (comGF) [Staphylococcus aureus isolate 7_4623]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1149338 DXE63_RS09710 WP_001825340.1 1835619..1836116(+) (comGF) [Staphylococcus aureus isolate 7_4623]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment