Detailed information NTG1148762

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: C6H44_RS18470
• Genome accession: NZ_LT963391
• Coordinates: 3891308..3892180 (+)
• Length: 290 a.a.
• NCBI ID: WP_010216946.1
• Uniprot ID: A0A0Q0CUR9
• Organism: Pseudomonas syringae pv. cerasicola isolate CFBP6109
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3886308..3897180

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H44_RS18450 (CFBP6109_03679)	-	3886514..3886948 (-)	435	WP_080393789.1	pilin	-
C6H44_RS18455 (CFBP6109_03680)	-	3887114..3888091 (+)	978	WP_032701292.1	IS5 family transposase	-
C6H44_RS18460 (CFBP6109_03681)	pilB	3888392..3890086 (+)	1695	WP_044320764.1	type IV-A pilus assembly ATPase PilB	Machinery gene
C6H44_RS18465 (CFBP6109_03682)	pilC	3890089..3891306 (+)	1218	WP_044320765.1	type II secretion system F family protein	Machinery gene
C6H44_RS18470 (CFBP6109_03683)	pilD	3891308..3892180 (+)	873	WP_010216946.1	prepilin peptidase	Machinery gene
C6H44_RS18475 (CFBP6109_03684)	coaE	3892177..3892800 (+)	624	WP_057460426.1	dephospho-CoA kinase	-
C6H44_RS18480 (CFBP6109_03685)	yacG	3892797..3893006 (+)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
C6H44_RS18485 (CFBP6109_03687)	-	3893204..3893476 (-)	273	Protein_3498	hypothetical protein	-
C6H44_RS18490 (CFBP6109_03688)	-	3893561..3894190 (+)	630	WP_002553210.1	DUF1780 domain-containing protein	-
C6H44_RS18495 (CFBP6109_03689)	-	3894187..3894720 (+)	534	WP_002553209.1	MOSC domain-containing protein	-
C6H44_RS18505 (CFBP6109_03690)	-	3894938..3896236 (+)	1299	WP_044321605.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32108.25 Da
• Isoelectric Point: 8.5797

>NTDB_id=1148762 C6H44_RS18470 WP_010216946.1 3891308..3892180(+) (pilD) [Pseudomonas syringae pv. cerasicola isolate CFBP6109]
MPLLDFLASSPLAFVTTCCILGLIIGSFLNVVVYRLPKMMERDWKAQSREMLGLPAEPDQPAFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQTAAMLVLSWGLLTMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1148762 C6H44_RS18470 WP_010216946.1 3891308..3892180(+) (pilD) [Pseudomonas syringae pv. cerasicola isolate CFBP6109]
ATGCCCCTCCTCGATTTCCTAGCCAGCTCCCCTCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTCCCCAAAATGATGGAGCGCGACTGGAAAGCCCAATCCCGCGAAATGCTCGGCC
TGCCCGCGGAACCCGATCAGCCCGCCTTCAACCTGAACCGCCCTCGTTCCAGCTGCCCGCACTGCGCGCACAAAATCCGC
CCGTGGGAAAACCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGTAAATGCTCACAATGCAAAGCCCCCATCAGCAAACG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGTTTCGGCTGGCAAACGGCCG
CGATGCTGGTGTTGAGCTGGGGCCTGCTGACGATGAGTCTGATCGACGCCGATCACCAGTTACTCCCTGACTCTCTGGTA
CTGCCACTGCTGTGGCTAGGCCTCATCGTCAACGCTTTCGGGCTGTTCACCTCGCTGAACGATGCGCTTTGGGGTGCGGT
GGCCGGTTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCATGGGGCGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCATCGCTGGTGGGC
GCGGTGCTTGGGGTGATAATGATGCGCGTGCGCAGGGTTGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: A0A0Q0CUR9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.986	95.172	0.514
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	51.056	97.931	0.5
pilD	Acinetobacter nosocomialis M2	50	97.931	0.49
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476