Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   CKV88_RS00210 Genome accession   NZ_LT906460
Coordinates   46416..47750 (-) Length   444 a.a.
NCBI ID   WP_002464576.1    Uniprot ID   G5JK73
Organism   Staphylococcus simiae strain NCTC13838     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 41416..52750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV88_RS00180 (SAMEA4384339_00038) - 42327..42773 (-) 447 WP_002464569.1 RadC family protein -
  CKV88_RS00185 (SAMEA4384339_00039) - 42770..42976 (-) 207 WP_002464570.1 hypothetical protein -
  CKV88_RS00190 (SAMEA4384339_00040) - 42996..43505 (-) 510 WP_002464571.1 DUF1643 domain-containing protein -
  CKV88_RS00195 (SAMEA4384339_00041) - 43522..43833 (-) 312 WP_002464572.1 DUF960 family protein -
  CKV88_RS00200 (SAMEA4384339_00043) - 43922..44269 (-) 348 WP_002464574.1 SAUGI family uracil-DNA glycosylase inhibitor -
  CKV88_RS00205 (SAMEA4384339_00044) ccrB 44755..46380 (-) 1626 WP_002464575.1 cassette chromosome recombinase CcrB Machinery gene
  CKV88_RS00210 (SAMEA4384339_00045) ccrA 46416..47750 (-) 1335 WP_002464576.1 cassette chromosome recombinase CcrA Machinery gene
  CKV88_RS00215 (SAMEA4384339_00046) - 47919..48197 (-) 279 WP_002464577.1 hypothetical protein -
  CKV88_RS00220 (SAMEA4384339_00047) cch1 48276..50066 (-) 1791 WP_002464578.1 cassette chromosome replicative helicase -
  CKV88_RS00225 (SAMEA4384339_00048) - 50066..50338 (-) 273 WP_002464579.1 hypothetical protein -
  CKV88_RS00230 (SAMEA4384339_00049) ssb 50378..50629 (-) 252 WP_002464580.1 cassette chromosome ssDNA-binding protein -
  CKV88_RS00235 (SAMEA4384339_00050) - 50766..51803 (+) 1038 WP_002464581.1 hypothetical protein -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 52077.01 Da        Isoelectric Point: 10.0395

>NTDB_id=1147622 CKV88_RS00210 WP_002464576.1 46416..47750(-) (ccrA) [Staphylococcus simiae strain NCTC13838]
MTQAIGYLRQSTLKQQSLTTQKNMIELLAKKHHIQHITFFSDKQSGRNDDRSGYQQMIELIQKEQCNVLCCYRLNRLHRN
LKNALKLVKLCQKHHVHILSVYDGFFNMNQSFDRLKLNVFISLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYRYH
NGTFTINKKEALTVKAVFDYYIQGFGYKKIAQFLEENHYLIDRKPYQVRNIIINPNYCGRVINQYGQFDNIFTAIISTTV
YQQAQNIRTNKQLKCEPSNNQLKQKIKCPFCDSVLTNLTIRKRNHSLRYYVCPKNMNQAQHCCPFTTINAKDLEIEVLNI
CRNFFQNKHVYQMLQDAIRTKLKNNQQKSTKSKMTQAQLIDKLAQGAIDIDTFRKQTSTISQENKKISKINDTQLQEAFK
NVITQYFTLNTLYPYIEEIHINQMKQVIGIYFKNEPLNIVNQNK

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=1147622 CKV88_RS00210 WP_002464576.1 46416..47750(-) (ccrA) [Staphylococcus simiae strain NCTC13838]
TTGACACAAGCAATTGGTTACTTACGTCAAAGTACATTAAAACAACAATCACTCACTACTCAAAAAAATATGATAGAGCT
ATTAGCTAAAAAACATCATATTCAACACATCACCTTTTTTAGTGATAAACAATCCGGTAGAAATGATGATAGAAGTGGAT
ATCAACAAATGATCGAACTCATTCAAAAGGAACAATGTAACGTACTATGTTGTTATCGCTTAAATCGATTACATCGAAAT
CTAAAAAATGCTTTAAAACTTGTAAAATTATGTCAAAAACATCACGTTCATATACTCAGTGTATACGATGGATTCTTCAA
TATGAATCAATCATTTGATCGTCTTAAGCTCAACGTTTTTATAAGTTTGGCAGAGCTTGAATCCGATAATATTGGAGAAC
AGGTCAAAAATGGTCTCAGAGAAAAAGCCAAACAAGGTAAACTGATTACGACACATGCCCCTTTTGGCTATCGCTATCAT
AATGGTACTTTTACAATCAATAAAAAAGAAGCACTGACTGTCAAAGCTGTATTCGACTACTATATTCAAGGCTTTGGCTA
TAAAAAGATTGCTCAATTCTTAGAAGAAAATCATTACCTTATCGACAGAAAGCCTTATCAAGTTCGAAATATCATCATCA
ATCCTAATTATTGTGGTCGTGTCATCAATCAATATGGACAATTTGACAATATATTTACAGCTATTATTAGTACTACTGTA
TATCAACAAGCTCAAAATATACGGACTAATAAGCAACTCAAGTGTGAACCATCAAATAATCAGCTAAAACAAAAAATAAA
ATGTCCATTTTGTGATTCAGTTCTCACAAATTTGACGATAAGAAAAAGAAATCATTCATTACGTTATTATGTTTGTCCTA
AAAATATGAATCAAGCTCAACATTGCTGTCCATTCACGACCATCAATGCTAAAGACCTTGAAATAGAAGTACTGAATATT
TGTAGAAACTTCTTTCAAAATAAACATGTCTATCAAATGTTACAAGACGCTATTAGAACAAAACTCAAAAATAACCAACA
AAAATCTACTAAAAGTAAAATGACTCAAGCACAATTGATAGATAAGTTAGCCCAAGGTGCTATCGACATTGATACTTTTA
GAAAACAGACATCAACAATTTCCCAAGAAAATAAAAAAATATCAAAAATAAACGATACTCAGCTACAAGAGGCTTTCAAA
AATGTGATTACTCAATATTTTACATTGAATACATTATATCCTTATATTGAGGAAATCCATATTAATCAAATGAAACAAGT
TATTGGTATTTACTTTAAAAATGAGCCGCTAAATATCGTCAATCAAAATAAATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-249)

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G5JK73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

67.421

99.55

0.671
  ccrA Staphylococcus aureus N315

65.837

99.55

0.655

Multiple sequence alignment