Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   CKV88_RS00205 Genome accession   NZ_LT906460
Coordinates   44755..46380 (-) Length   541 a.a.
NCBI ID   WP_002464575.1    Uniprot ID   G5JK72
Organism   Staphylococcus simiae strain NCTC13838     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 39755..51380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV88_RS00165 (SAMEA4384339_00035) - 40429..40923 (-) 495 WP_002464566.1 hypothetical protein -
  CKV88_RS00170 (SAMEA4384339_00036) - 40939..41229 (-) 291 WP_002464567.1 hypothetical protein -
  CKV88_RS00175 (SAMEA4384339_00037) - 41280..42158 (-) 879 WP_002464568.1 HTH domain-containing protein -
  CKV88_RS00180 (SAMEA4384339_00038) - 42327..42773 (-) 447 WP_002464569.1 RadC family protein -
  CKV88_RS00185 (SAMEA4384339_00039) - 42770..42976 (-) 207 WP_002464570.1 hypothetical protein -
  CKV88_RS00190 (SAMEA4384339_00040) - 42996..43505 (-) 510 WP_002464571.1 DUF1643 domain-containing protein -
  CKV88_RS00195 (SAMEA4384339_00041) - 43522..43833 (-) 312 WP_002464572.1 DUF960 family protein -
  CKV88_RS00200 (SAMEA4384339_00043) - 43922..44269 (-) 348 WP_002464574.1 SAUGI family uracil-DNA glycosylase inhibitor -
  CKV88_RS00205 (SAMEA4384339_00044) ccrB 44755..46380 (-) 1626 WP_002464575.1 cassette chromosome recombinase CcrB Machinery gene
  CKV88_RS00210 (SAMEA4384339_00045) ccrA 46416..47750 (-) 1335 WP_002464576.1 cassette chromosome recombinase CcrA Machinery gene
  CKV88_RS00215 (SAMEA4384339_00046) - 47919..48197 (-) 279 WP_002464577.1 hypothetical protein -
  CKV88_RS00220 (SAMEA4384339_00047) cch1 48276..50066 (-) 1791 WP_002464578.1 cassette chromosome replicative helicase -
  CKV88_RS00225 (SAMEA4384339_00048) - 50066..50338 (-) 273 WP_002464579.1 hypothetical protein -
  CKV88_RS00230 (SAMEA4384339_00049) ssb 50378..50629 (-) 252 WP_002464580.1 cassette chromosome ssDNA-binding protein -

Sequence

Protein


Download         Length: 541 a.a.        Molecular weight: 62535.77 Da        Isoelectric Point: 9.9143

>NTDB_id=1147621 CKV88_RS00205 WP_002464575.1 44755..46380(-) (ccrB) [Staphylococcus simiae strain NCTC13838]
MQYKQKRVGIYVRVSTEMQSNEGYSIEGQISQIKEYCHFNNFVVADVYADRGISGKSMNRPELQRMLKDAKDGNLDCIMV
YKTNRLARNTSDLLKIVEDLHRLNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGNL
PLGYDKVPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSINAITYILTNPFYIGKIRFAKY
KDWNEKRRKGLNDKPIIAEGKHSAIISHELWEKVQYRKKQVSKKPQVHGKGTNLLTGIIRCPQCGAPMAASNTTNTLKDG
TKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMQKLLEIVQKDKIIKQVVERINQTPQVDKAALNHDIAYKQQQFDE
IRTKQTNLIKAIEDNPDITSILEETINQYEGQLKTITEQMNQLKHEQTKEISTFDTNKIASILQHIFKNIGTMEKSQLKR
LYLTVIDHIDIRKSNQSKKQFYVTLKLNNDIINQLFNNQPIGEVLLCTSPIFLSNTLYFQF

Nucleotide


Download         Length: 1626 bp        

>NTDB_id=1147621 CKV88_RS00205 WP_002464575.1 44755..46380(-) (ccrB) [Staphylococcus simiae strain NCTC13838]
ATGCAATATAAACAAAAACGTGTTGGTATTTATGTACGTGTATCTACAGAGATGCAAAGTAACGAAGGTTACAGTATTGA
AGGGCAAATCAGTCAAATTAAAGAGTATTGTCATTTTAATAATTTTGTCGTAGCAGATGTATATGCCGATCGAGGTATTT
CTGGAAAATCTATGAACCGTCCTGAATTACAACGCATGTTGAAAGATGCGAAAGATGGTAATTTGGACTGTATTATGGTT
TACAAAACCAATCGACTTGCTAGAAATACTTCTGATTTGTTGAAAATAGTTGAAGATTTACATCGACTAAACGTTGAGTT
TTTCAGTCTATCTGAACGTATGGAAGTGAATACGAGTAGTGGTAAACTTATGCTTCAAATCTTAGCATCTTTTTCAGAAT
TTGAAAGAAATAATATTGTAGAAAATGTCTTTATGGGGCAAACACGACGTGCTCAAGAAGGTTATTATCAAGGAAATCTA
CCTCTAGGATATGATAAAGTTCCTGATAGTAAACATGAACTCATGATTAATCAACATGAAGCTAATATTGTCAAATATAT
ATTTGAATCCTATGCTAAAGGACATGGCTATCGTAAAATAGCGAATGCTTTGAACCACAAAGGTTATGTGACTAAGAAAG
GAAAACCTTTTAGTATTAATGCTATTACTTATATACTTACTAATCCATTCTATATTGGTAAAATTAGGTTTGCGAAATAC
AAAGATTGGAATGAAAAACGTCGTAAAGGACTTAATGATAAACCCATTATTGCTGAAGGTAAGCACTCTGCTATTATTAG
CCACGAACTTTGGGAGAAAGTTCAATATAGAAAAAAACAAGTGAGTAAAAAACCTCAAGTCCACGGTAAAGGAACAAATT
TACTAACTGGTATTATTCGATGTCCACAATGTGGAGCTCCTATGGCTGCTAGTAATACAACTAATACACTGAAAGATGGT
ACTAAAAAGCGTATTCGCTATTATTCTTGCAGTAATTTTCGTAATAAAGGATCCAAAGTGTGTTCTGCCAATAGTGTTAG
AGCAGATGTAATAGAAGACTATGTTATGCAAAAACTACTGGAAATAGTCCAAAAGGATAAAATAATTAAACAAGTTGTCG
AACGTATCAATCAAACGCCACAAGTTGATAAAGCAGCATTGAATCATGATATTGCCTATAAACAACAACAGTTTGATGAA
ATACGTACCAAGCAAACTAACTTAATCAAAGCAATTGAAGATAATCCAGATATAACATCTATACTTGAAGAAACAATAAA
CCAATATGAAGGTCAATTAAAGACAATTACAGAACAAATGAATCAACTCAAACATGAACAAACGAAAGAGATTTCAACTT
TTGATACTAATAAAATTGCTTCTATCCTTCAACATATATTCAAAAATATAGGAACTATGGAGAAATCACAACTCAAAAGA
TTATATCTTACTGTTATTGACCATATTGATATTAGAAAAAGCAATCAATCTAAAAAGCAATTCTATGTTACTCTCAAGCT
GAATAATGATATCATCAATCAACTTTTTAATAACCAACCTATAGGCGAAGTGCTTCTCTGCACTTCGCCTATATTTTTAT
CTAATACACTATACTTTCAATTTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(179-279)

Resolvase, N terminal domain (Resolvase)

(10-155)

Ogr/Delta-like zinc finger (Ogr_Delta)

(300-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G5JK72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

79.554

99.445

0.791

Multiple sequence alignment