Detailed information NTG1147223

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: CKW04_RS04710
• Genome accession: NZ_LT906442
• Coordinates: 970405..970845 (-)
• Length: 146 a.a.
• NCBI ID: WP_231951044.1
• Uniprot ID: A0A0W1A0G0
• Organism: Legionella waltersii strain NCTC13017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 965405..975845

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKW04_RS04695 (SAMEA4504053_00957)	-	966393..967283 (-)	891	WP_231951018.1	class I SAM-dependent methyltransferase	-
CKW04_RS04700 (SAMEA4504053_00958)	-	967291..967920 (-)	630	WP_058481380.1	hypothetical protein	-
CKW04_RS04705 (SAMEA4504053_00959)	comEC	968078..970279 (-)	2202	WP_058481381.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CKW04_RS04710 (SAMEA4504053_00960)	pilE	970405..970845 (-)	441	WP_231951044.1	type IV pilin protein	Machinery gene
CKW04_RS04715 (SAMEA4504053_00961)	-	970861..974361 (-)	3501	WP_058481382.1	pilus assembly protein	-
CKW04_RS04720 (SAMEA4504053_00962)	-	974374..974883 (-)	510	WP_058481383.1	pilus assembly protein	-

Sequence

Protein

• Length: 146 a.a.
• Molecular weight: 16095.42 Da
• Isoelectric Point: 6.1936

>NTDB_id=1147223 CKW04_RS04710 WP_231951044.1 970405..970845(-) (pilE) [Legionella waltersii strain NCTC13017]
MNIGMKKQNINRELGFSLIELMIAMVILGILTAIAYPSYKQYIMESRRSDALSTLTQDQIIFERCYSQNFSYAAACGAIP
VFPQTSPQGYYRITATNVTPTTYTLTATPLGTQVEDTDCVSMSINQANVKTALDSSAASQPKCWNP

Nucleotide

• Length: 441 bp

>NTDB_id=1147223 CKW04_RS04710 WP_231951044.1 970405..970845(-) (pilE) [Legionella waltersii strain NCTC13017]
ATGAACATTGGAATGAAAAAACAAAACATCAACAGGGAGTTAGGGTTTTCATTAATTGAATTGATGATAGCCATGGTTAT
CTTAGGGATTTTGACTGCTATTGCCTACCCCTCGTATAAACAATACATCATGGAGTCAAGACGTTCTGATGCCCTAAGTA
CATTAACTCAAGATCAAATCATTTTTGAACGCTGCTACTCGCAAAATTTTTCATATGCGGCAGCTTGTGGTGCAATTCCA
GTCTTTCCTCAAACCTCTCCACAGGGATATTACAGAATTACAGCCACTAACGTAACTCCAACAACCTACACCTTAACCGC
GACACCTCTTGGTACACAAGTCGAGGATACGGATTGTGTGAGTATGTCTATTAATCAAGCCAATGTTAAAACTGCATTGG
ACTCTTCCGCAGCATCCCAACCTAAATGTTGGAACCCATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (11-36)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (37-122)

3D structure

• AlphaFold DB: A0A0W1A0G0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	67.586	99.315	0.671
pilE	Legionella pneumophila strain ERS1305867	67.586	99.315	0.671