Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VCBK1071_RS01535 Genome accession   NZ_LT897797
Coordinates   320964..321548 (+) Length   194 a.a.
NCBI ID   WP_172440033.1    Uniprot ID   -
Organism   Vibrio cholerae isolate Vibrio cholerae str. BC1071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315964..326548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCBK1071_RS01520 (VCBK1071_00293) oxyR 316370..317263 (+) 894 WP_101410075.1 DNA-binding transcriptional regulator OxyR -
  VCBK1071_RS01525 (VCBK1071_00294) - 317338..319815 (-) 2478 WP_000668738.1 penicillin-binding protein 1A -
  VCBK1071_RS01530 (VCBK1071_00295) pilM 319958..320974 (+) 1017 WP_095471438.1 type IV pilus assembly protein PilM Machinery gene
  VCBK1071_RS01535 (VCBK1071_00296) pilN 320964..321548 (+) 585 WP_172440033.1 PilN domain-containing protein Machinery gene
  VCBK1071_RS01540 (VCBK1071_00297) pilO 321541..322131 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCBK1071_RS01545 (VCBK1071_00298) pilP 322121..322639 (+) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  VCBK1071_RS01550 (VCBK1071_00299) pilQ 322665..324380 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  VCBK1071_RS01555 (VCBK1071_00300) aroK 324567..325091 (+) 525 WP_000818616.1 shikimate kinase AroK -
  VCBK1071_RS01560 (VCBK1071_00301) aroB 325119..326204 (+) 1086 WP_172440034.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22056.51 Da        Isoelectric Point: 9.9274

>NTDB_id=1146648 VCBK1071_RS01535 WP_172440033.1 320964..321548(+) (pilN) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQFLTPASNPKEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=1146648 VCBK1071_RS01535 WP_172440033.1 320964..321548(+) (pilN) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCCGGTGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTGCAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACAGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAATTTTTAACCCCAGCCTCTAACCCGAA
AGAGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

98.454

100

0.985
  pilN Vibrio campbellii strain DS40M4

60.335

92.268

0.557

Multiple sequence alignment