Detailed information NTG1146647

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VCBK1071_RS01530
• Genome accession: NZ_LT897797
• Coordinates: 319958..320974 (+)
• Length: 338 a.a.
• NCBI ID: WP_095471438.1
• Uniprot ID: -
• Organism: Vibrio cholerae isolate Vibrio cholerae str. BC1071
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 314958..325974

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VCBK1071_RS01515 (VCBK1071_00292)	-	315499..316230 (-)	732	WP_000495226.1	glutathione peroxidase	-
VCBK1071_RS01520 (VCBK1071_00293)	oxyR	316370..317263 (+)	894	WP_101410075.1	DNA-binding transcriptional regulator OxyR	-
VCBK1071_RS01525 (VCBK1071_00294)	-	317338..319815 (-)	2478	WP_000668738.1	penicillin-binding protein 1A	-
VCBK1071_RS01530 (VCBK1071_00295)	pilM	319958..320974 (+)	1017	WP_095471438.1	type IV pilus assembly protein PilM	Machinery gene
VCBK1071_RS01535 (VCBK1071_00296)	pilN	320964..321548 (+)	585	WP_172440033.1	PilN domain-containing protein	Machinery gene
VCBK1071_RS01540 (VCBK1071_00297)	pilO	321541..322131 (+)	591	WP_000157688.1	type 4a pilus biogenesis protein PilO	Machinery gene
VCBK1071_RS01545 (VCBK1071_00298)	pilP	322121..322639 (+)	519	WP_000792033.1	pilus assembly protein PilP	Machinery gene
VCBK1071_RS01550 (VCBK1071_00299)	pilQ	322665..324380 (+)	1716	WP_000788426.1	type IV pilus secretin PilQ	Machinery gene
VCBK1071_RS01555 (VCBK1071_00300)	aroK	324567..325091 (+)	525	WP_000818616.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37733.47 Da
• Isoelectric Point: 7.2989

>NTDB_id=1146647 VCBK1071_RS01530 WP_095471438.1 319958..320974(+) (pilM) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVYGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=1146647 VCBK1071_RS01530 WP_095471438.1 319958..320974(+) (pilM) [Vibrio cholerae isolate Vibrio cholerae str. BC1071]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGTACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGTGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	99.704	100	0.997
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485