Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   B5474_RS17400 Genome accession   NZ_LT605059
Coordinates   3638227..3638967 (-) Length   246 a.a.
NCBI ID   WP_000076099.1    Uniprot ID   A0A9P2QDS6
Organism   Acinetobacter baumannii strain NCTC7364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3633227..3643967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5474_RS17380 (NCTC7364_03483) aroB 3633769..3634851 (-) 1083 WP_038405376.1 3-dehydroquinate synthase -
  B5474_RS17385 (NCTC7364_03484) aroK 3634880..3635449 (-) 570 WP_000643712.1 shikimate kinase AroK -
  B5474_RS17390 (NCTC7364_03485) pilQ 3635461..3637626 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  B5474_RS17395 (NCTC7364_03486) pilP 3637689..3638216 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  B5474_RS17400 (NCTC7364_03487) pilO 3638227..3638967 (-) 741 WP_000076099.1 type 4a pilus biogenesis protein PilO Machinery gene
  B5474_RS17405 (NCTC7364_03488) pilN 3638964..3639605 (-) 642 WP_000201224.1 PilN domain-containing protein Machinery gene
  B5474_RS17410 (NCTC7364_03489) pilM 3639605..3640663 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  B5474_RS17415 (NCTC7364_03490) ponA 3640825..3643380 (+) 2556 WP_079549043.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27801.55 Da        Isoelectric Point: 4.8357

>NTDB_id=1145167 B5474_RS17400 WP_000076099.1 3638227..3638967(-) (pilO) [Acinetobacter baumannii strain NCTC7364]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTIHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1145167 B5474_RS17400 WP_000076099.1 3638227..3638967(-) (pilO) [Acinetobacter baumannii strain NCTC7364]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATTCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment