Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LLA12_RS05500 Genome accession   NZ_LT599049
Coordinates   1132459..1133781 (-) Length   440 a.a.
NCBI ID   WP_021722513.1    Uniprot ID   S6FT74
Organism   Lactococcus lactis subsp. lactis strain A12     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1127459..1138781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLA12_RS05470 (LLA12_01158) - 1128602..1129570 (+) 969 WP_021722518.1 PhoH family protein -
  LLA12_RS05475 (LLA12_01159) - 1129628..1130107 (+) 480 WP_021722517.1 NUDIX hydrolase -
  LLA12_RS05480 (LLA12_01160) ybeY 1130259..1130747 (+) 489 WP_021722516.1 rRNA maturation RNase YbeY -
  LLA12_RS05485 (LLA12_01161) - 1130731..1131186 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  LLA12_RS05490 (LLA12_01162) - 1131200..1131778 (+) 579 WP_021722515.1 nucleotidyltransferase family protein -
  LLA12_RS14100 comFC 1131812..1132216 (-) 405 WP_021722514.1 ComF family protein Machinery gene
  LLA12_RS05500 (LLA12_01164) comFA 1132459..1133781 (-) 1323 WP_021722513.1 DEAD/DEAH box helicase Machinery gene
  LLA12_RS05505 (LLA12_01165) - 1133838..1134467 (+) 630 WP_003130660.1 YigZ family protein -
  LLA12_RS05510 (LLA12_01166) - 1134601..1135098 (+) 498 WP_012897708.1 VanZ family protein -
  LLA12_RS05515 (LLA12_01167) - 1135146..1136165 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  LLA12_RS05520 (LLA12_01168) - 1136281..1137027 (+) 747 WP_021722512.1 bifunctional metallophosphatase/5'-nucleotidase -
  LLA12_RS05525 (LLA12_01170) glyQ 1137312..1138265 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50150.67 Da        Isoelectric Point: 10.1468

>NTDB_id=1144865 LLA12_RS05500 WP_021722513.1 1132459..1133781(-) (comFA) [Lactococcus lactis subsp. lactis strain A12]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNTILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRKSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILLATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=1144865 LLA12_RS05500 WP_021722513.1 1132459..1133781(-) (comFA) [Lactococcus lactis subsp. lactis strain A12]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATACAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAACAAATTTTAGAAAGC
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCACGTCGTTTTCACAATTTTCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAAGAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTACTAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-232)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6FT74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.5

90.909

0.677
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.251

100

0.382

Multiple sequence alignment