Detailed information NTG1143479

Bioinformatically predicted

Overview

• Name: pilW
• Type: Machinery gene
• Locus tag: AC2117_RS01700
• Genome accession: NZ_LS999521
• Coordinates: 364504..365505 (+)
• Length: 333 a.a.
• NCBI ID: WP_133971523.1
• Uniprot ID: A0A446ZFF0
• Organism: Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 359504..370505

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AC2117_RS01670	-	361081..361170 (-)	90	Protein_318	hypothetical protein	-
AC2117_RS01675 (AC2117_00331)	rpoZ	361251..361529 (-)	279	WP_002116990.1	DNA-directed RNA polymerase subunit omega	-
AC2117_RS01680 (AC2117_00332)	gmk	361602..362231 (-)	630	WP_042895429.1	guanylate kinase	-
AC2117_RS01685 (AC2117_00333)	ispH	362354..363304 (+)	951	WP_042895425.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
AC2117_RS01690 (AC2117_00334)	fimU	363477..363950 (+)	474	WP_133971519.1	Tfp pilus assembly protein FimT/FimU	Machinery gene
AC2117_RS01695 (AC2117_00335)	pilV	363944..364504 (+)	561	WP_133971521.1	type IV pilus modification protein PilV	Machinery gene
AC2117_RS01700 (AC2117_00336)	pilW	364504..365505 (+)	1002	WP_133971523.1	PilW family protein	Machinery gene
AC2117_RS01705 (AC2117_00337)	pilX	365502..366320 (+)	819	WP_197730966.1	PilX N-terminal domain-containing pilus assembly protein	Machinery gene
AC2117_RS01710 (AC2117_00338)	pilY1	366332..370186 (+)	3855	WP_197730967.1	PilC/PilY family type IV pilus protein	Machinery gene

Sequence

Protein

• Length: 333 a.a.
• Molecular weight: 35800.57 Da
• Isoelectric Point: 6.6395

>NTDB_id=1143479 AC2117_RS01700 WP_133971523.1 364504..365505(+) (pilW) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
MTHSFVSWKQRGFTLIELMVALALGLILVAAATQLFIGGLLSSRLQKANAEIQDSGIFGLEYIARDIRLLNYGNVINPIL
TDTTPWGGVVLTGSTATNNDSVNFVPNVGTNTYIAETLLSRGVGDTVSTVSNHWKGLSNIQNSSNAAMQSDQLTIQFIAP
TNMTNCEGVNVLAGDLIVERYFLRLDTNGSSQQDYALACDANTPSSVATAQPTTVSGLGDAGQIILPRIDHFHVLFGAKN
AAGNFAYYTIPQYRAAAQAARDATPPVMAPRILSIQISVLARSTNNAQNKAIDPTQSFLMLDQTVHASDNTTRFLRRVYS
VTIALRNAMGESL

Nucleotide

• Length: 1002 bp

>NTDB_id=1143479 AC2117_RS01700 WP_133971523.1 364504..365505(+) (pilW) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
ATGACTCACTCATTTGTATCGTGGAAGCAACGAGGCTTTACATTAATTGAGCTTATGGTTGCATTGGCTTTAGGCCTAAT
TTTGGTCGCTGCGGCAACTCAATTATTTATTGGTGGACTTCTATCAAGCCGCTTGCAAAAAGCAAATGCTGAAATACAAG
ATAGCGGTATTTTTGGCTTGGAATATATTGCACGTGATATACGTCTTCTAAATTATGGAAATGTAATAAATCCAATATTA
ACGGATACCACGCCTTGGGGAGGTGTTGTATTAACTGGTTCAACAGCAACTAATAATGACAGTGTCAATTTTGTTCCTAA
TGTTGGCACAAATACCTATATTGCTGAGACACTATTAAGTCGTGGTGTGGGAGATACGGTAAGTACCGTTAGTAATCACT
GGAAAGGCCTTTCAAATATACAGAATAGTTCAAATGCTGCTATGCAAAGCGATCAGCTGACTATTCAGTTTATTGCTCCT
ACAAATATGACAAATTGTGAAGGCGTGAATGTACTTGCTGGAGATTTAATTGTAGAGCGGTATTTTTTACGTCTTGATAC
GAATGGTAGTTCTCAGCAAGATTATGCGCTTGCATGTGATGCAAATACTCCCTCTAGCGTAGCTACGGCACAACCTACCA
CTGTGTCTGGATTAGGAGATGCTGGTCAAATTATATTGCCGCGTATTGACCATTTCCATGTGTTATTCGGCGCAAAGAAT
GCTGCTGGCAATTTTGCTTATTACACAATTCCCCAATATCGGGCTGCCGCACAGGCTGCCCGAGATGCCACTCCACCAGT
AATGGCACCTAGAATTCTTTCAATACAAATATCGGTGTTAGCCCGTTCTACTAACAATGCCCAAAATAAAGCAATAGATC
CCACACAGTCGTTTCTTATGTTGGATCAAACTGTACATGCTTCTGATAACACAACTCGATTTTTGCGCCGTGTTTATAGC
GTAACAATTGCTCTACGTAATGCGATGGGGGAAAGTCTATGA

Domains

Predicted by InterproScan.

• Type IV Pilus-assembly protein W (PilW) (181-327)

• Prokaryotic N-terminal methylation motif (N_methyl) (9-32)

3D structure

• AlphaFold DB: A0A446ZFF0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilW	Acinetobacter baumannii D1279779	86.787	100	0.868