Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AC2117_RS01580 Genome accession   NZ_LS999521
Coordinates   338671..339420 (+) Length   249 a.a.
NCBI ID   WP_133971491.1    Uniprot ID   A0A446ZFD1
Organism   Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333671..344420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC2117_RS01565 (AC2117_00310) ponA 334252..336813 (-) 2562 WP_133971485.1 penicillin-binding protein PBP1a -
  AC2117_RS01570 (AC2117_00311) pilM 336975..338033 (+) 1059 WP_133971487.1 pilus assembly protein PilM Machinery gene
  AC2117_RS01575 (AC2117_00312) pilN 338033..338674 (+) 642 WP_133971489.1 PilN domain-containing protein Machinery gene
  AC2117_RS01580 (AC2117_00313) pilO 338671..339420 (+) 750 WP_133971491.1 type 4a pilus biogenesis protein PilO Machinery gene
  AC2117_RS01585 (AC2117_00314) pilP 339431..339958 (+) 528 WP_133971492.1 pilus assembly protein PilP Machinery gene
  AC2117_RS01590 (AC2117_00315) pilQ 340021..342186 (+) 2166 WP_133971494.1 type IV pilus secretin PilQ family protein Machinery gene
  AC2117_RS01595 (AC2117_00316) aroK 342198..342767 (+) 570 WP_042895460.1 shikimate kinase AroK -
  AC2117_RS01600 (AC2117_00317) aroB 342796..343878 (+) 1083 WP_133971496.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28122.94 Da        Isoelectric Point: 4.7637

>NTDB_id=1143473 AC2117_RS01580 WP_133971491.1 338671..339420(+) (pilO) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWVFIFFAVLALGYFVVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQDMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITMEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPDKDSKSDIPVLNYSIKAKTYRYMGAVENAEHLENNASASVAPV
SSTANTQSK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=1143473 AC2117_RS01580 WP_133971491.1 338671..339420(+) (pilO) [Acinetobacter calcoaceticus isolate Acinetobacter calcoaceticus str. 2117]
ATGAGTCCGGATGAACTTCATGAGTTGTCGTTAGAGCAGGCGCCATCTAAGAAAAAAAAGATCACTTTAGAAAAATTTCT
TCAGCAATTTAATACACTAGATATGAACAGTTATGGTAGCTGGCCATTATCTGTGAAAATTACTTGCTGGGTTTTTATTT
TCTTTGCTGTGTTGGCTTTGGGATACTTTGTTGTTATTCAGCCAAAGCTACAAGCAATTGATAATGCACAGGCACAAGAA
AGTAATTTGTTGAATGAGTTTCGAGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAGCTTCAACTTCAAGATAT
GCAAGCAAACTTTAATCAGCAGTTAGAGCAATTGCCAAAAGAAACTGAAATTCCAAGCTTAGTTGAAGATATTAACTTAA
CTGGGGTGAATTCTGGCCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAAGAAATCTTTATTGAACAGCCT
ATTACGATGGAAGCAACCGGCGATTATCACGCTTTTGGTGCTTTTGTCAGTAGTATTGCGGCACTACCACGTATTGTGAC
CATGCATGATTTTGTTGTAGAAGCTGCACCTGACAAAGATAGTAAGTCAGATATTCCTGTACTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGCGCCGTAGAAAATGCTGAACATCTGGAAAACAACGCTTCAGCTTCAGTAGCACCTGTT
TCCTCTACTGCTAATACACAGTCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A446ZFD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

82.329

100

0.823
  comO Acinetobacter baylyi ADP1

68.952

99.598

0.687

Multiple sequence alignment