Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PA5486_RS28430 Genome accession   NZ_LS998783
Coordinates   5806076..5806948 (+) Length   290 a.a.
NCBI ID   WP_015648060.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5801076..5811948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA5486_RS28410 (PA5486_05523) - 5801356..5801820 (-) 465 WP_023129290.1 pilin -
  PA5486_RS28415 (PA5486_05524) pilB 5802051..5803751 (+) 1701 WP_030048379.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PA5486_RS28420 (PA5486_05525) - 5803822..5804838 (+) 1017 WP_032728035.1 IS30-like element IS1394 family transposase -
  PA5486_RS28425 (PA5486_05526) pilC 5804816..5806075 (+) 1260 WP_134163279.1 type II secretion system F family protein Machinery gene
  PA5486_RS28430 (PA5486_05527) pilD 5806076..5806948 (+) 873 WP_015648060.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PA5486_RS28435 (PA5486_05528) coaE 5806945..5807556 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PA5486_RS28440 (PA5486_05529) yacG 5807553..5807753 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PA5486_RS28445 (PA5486_05530) - 5807790..5807999 (-) 210 WP_003094660.1 hypothetical protein -
  PA5486_RS28450 (PA5486_05531) - 5808105..5808794 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PA5486_RS28455 (PA5486_05532) - 5808791..5809261 (-) 471 WP_003103866.1 hypothetical protein -
  PA5486_RS28460 (PA5486_05533) - 5809258..5809683 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PA5486_RS28465 (PA5486_05534) - 5809816..5810445 (+) 630 WP_015648058.1 DUF1780 domain-containing protein -
  PA5486_RS28470 (PA5486_05535) - 5810442..5810891 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  PA5486_RS28475 (PA5486_05536) - 5810917..5811087 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31889.86 Da        Isoelectric Point: 7.3454

>NTDB_id=1143389 PA5486_RS28430 WP_015648060.1 5806076..5806948(+) (pilD) [Pseudomonas aeruginosa isolate]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGNCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGTVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=1143389 PA5486_RS28430 WP_015648060.1 5806076..5806948(+) (pilD) [Pseudomonas aeruginosa isolate]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAACTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCACGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55

96.552

0.531
  pilD Vibrio campbellii strain DS40M4

51.943

97.586

0.507
  pilD Acinetobacter nosocomialis M2

50.177

97.586

0.49
  pilD Acinetobacter baumannii D1279779

50.177

97.586

0.49
  pilD Neisseria gonorrhoeae MS11

48.201

95.862

0.462

Multiple sequence alignment