Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQL12_RS11505 Genome accession   NZ_LS483450
Coordinates   2125033..2126331 (-) Length   432 a.a.
NCBI ID   WP_000867616.1    Uniprot ID   A0A0H2US77
Organism   Streptococcus pneumoniae strain 4041STDY6583227     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2120033..2131331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL12_RS11485 rplI 2121188..2121640 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  DQL12_RS11490 - 2121637..2123610 (-) 1974 WP_050255532.1 DHH family phosphoesterase -
  DQL12_RS11495 hpf 2123746..2124294 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DQL12_RS11500 comFC/cflB 2124374..2125036 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  DQL12_RS11505 comFA/cflA 2125033..2126331 (-) 1299 WP_000867616.1 DEAD/DEAH box helicase Machinery gene
  DQL12_RS11510 - 2126387..2127022 (+) 636 WP_000395742.1 YigZ family protein -
  DQL12_RS11515 cysK 2127307..2128227 (+) 921 WP_000029866.1 cysteine synthase A -
  DQL12_RS11525 - 2128302..2128840 (-) 539 Protein_2121 transposase -
  DQL12_RS11530 - 2128813..2129100 (+) 288 Protein_2122 transposase -
  DQL12_RS11535 tnpB 2129241..2129564 (-) 324 WP_000586643.1 IS66 family insertion sequence element accessory protein TnpB -
  DQL12_RS11540 tsf 2129810..2130850 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49590.61 Da        Isoelectric Point: 8.5992

>NTDB_id=1142023 DQL12_RS11505 WP_000867616.1 2125033..2126331(-) (comFA/cflA) [Streptococcus pneumoniae strain 4041STDY6583227]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1142023 DQL12_RS11505 WP_000867616.1 2125033..2126331(-) (comFA/cflA) [Streptococcus pneumoniae strain 4041STDY6583227]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCAAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGATTGCT
TCAAGTAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGCGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2US77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

100

100

1
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

52.451

94.444

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

39.211

99.769

0.391

Multiple sequence alignment