Detailed information NTG1141487

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQN29_RS00685
• Genome accession: NZ_LS483437
• Coordinates: 104906..105340 (+)
• Length: 144 a.a.
• NCBI ID: WP_002986542.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC13751
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99906..110340

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN29_RS00655 (NCTC13751_00133)	-	101575..101940 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQN29_RS00660 (NCTC13751_00134)	comYA	102033..102971 (+)	939	WP_002986557.1	competence type IV pilus ATPase ComGA	Machinery gene
DQN29_RS00665 (NCTC13751_00135)	comYB	102907..103941 (+)	1035	WP_228631569.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQN29_RS00670 (NCTC13751_00136)	comYC	103943..104269 (+)	327	WP_011528161.1	competence type IV pilus major pilin ComGC	Machinery gene
DQN29_RS00675 (NCTC13751_00137)	comGD	104244..104672 (+)	429	WP_076639399.1	competence type IV pilus minor pilin ComGD	-
DQN29_RS00680 (NCTC13751_00138)	comGE	104629..104913 (+)	285	WP_307777288.1	competence type IV pilus minor pilin ComGE	-
DQN29_RS00685 (NCTC13751_00139)	comYF	104906..105340 (+)	435	WP_002986542.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQN29_RS00690 (NCTC13751_00140)	comGG	105324..105650 (+)	327	WP_002986539.1	competence type IV pilus minor pilin ComGG	-
DQN29_RS00695 (NCTC13751_00141)	comYH	105748..106701 (+)	954	WP_010921802.1	class I SAM-dependent methyltransferase	Machinery gene
DQN29_RS00700 (NCTC13751_00142)	-	106760..107956 (+)	1197	WP_020904834.1	acetate kinase	-
DQN29_RS00705	-	108143..108451 (+)	309	Protein_90	hypothetical protein	-
DQN29_RS00710 (NCTC13751_00143)	proC	108534..109304 (-)	771	WP_076639397.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16573.20 Da
• Isoelectric Point: 10.3584

>NTDB_id=1141487 DQN29_RS00685 WP_002986542.1 104906..105340(+) (comYF) [Streptococcus pyogenes strain NCTC13751]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1141487 DQN29_RS00685 WP_002986542.1 104906..105340(+) (comYF) [Streptococcus pyogenes strain NCTC13751]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGCTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396