Detailed information NTG1140739

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQN44_RS00700
• Genome accession: NZ_LS483414
• Coordinates: 108249..108683 (+)
• Length: 144 a.a.
• NCBI ID: WP_002986542.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC13736
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 103249..113683

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN44_RS00670 (NCTC13736_00135)	-	104917..105282 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQN44_RS00675 (NCTC13736_00136)	comYA	105376..106314 (+)	939	WP_030127399.1	competence type IV pilus ATPase ComGA	Machinery gene
DQN44_RS00680 (NCTC13736_00137)	comYB	106250..107284 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQN44_RS00685 (NCTC13736_00138)	comYC	107286..107612 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQN44_RS00690 (NCTC13736_00139)	comGD	107587..108015 (+)	429	WP_030127397.1	competence type IV pilus minor pilin ComGD	-
DQN44_RS00695 (NCTC13736_00140)	comGE	107972..108256 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
DQN44_RS00700 (NCTC13736_00141)	comYF	108249..108683 (+)	435	WP_002986542.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQN44_RS00705 (NCTC13736_00142)	comGG	108667..108993 (+)	327	WP_002986539.1	competence type IV pilus minor pilin ComGG	-
DQN44_RS00710 (NCTC13736_00143)	comYH	109091..110044 (+)	954	WP_030127396.1	class I SAM-dependent methyltransferase	Machinery gene
DQN44_RS00715 (NCTC13736_00144)	-	110103..111299 (+)	1197	WP_011888536.1	acetate kinase	-
DQN44_RS00720	-	111486..111794 (+)	309	WP_030126458.1	hypothetical protein	-
DQN44_RS00725 (NCTC13736_00145)	proC	111877..112647 (-)	771	WP_030127395.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16573.20 Da
• Isoelectric Point: 10.3584

>NTDB_id=1140739 DQN44_RS00700 WP_002986542.1 108249..108683(+) (comYF) [Streptococcus pyogenes strain NCTC13736]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1140739 DQN44_RS00700 WP_002986542.1 108249..108683(+) (comYF) [Streptococcus pyogenes strain NCTC13736]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396