Detailed information NTG1137402

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQL04_RS00690
• Genome accession: NZ_LS483340
• Coordinates: 104787..105221 (+)
• Length: 144 a.a.
• NCBI ID: WP_002986542.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC12062
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99787..110221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL04_RS00660 (NCTC12062_00134)	-	101455..101820 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQL04_RS00665 (NCTC12062_00135)	comYA	101913..102851 (+)	939	WP_047235612.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL04_RS00670 (NCTC12062_00136)	comGB	102787..103822 (+)	1036	Protein_81	competence type IV pilus assembly protein ComGB	-
DQL04_RS00675 (NCTC12062_00137)	comYC	103824..104150 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL04_RS00680 (NCTC12062_00138)	comGD	104125..104553 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
DQL04_RS00685 (NCTC12062_00139)	comGE	104510..104794 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
DQL04_RS00690 (NCTC12062_00140)	comYF	104787..105221 (+)	435	WP_002986542.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL04_RS00695 (NCTC12062_00141)	comGG	105205..105531 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
DQL04_RS00700 (NCTC12062_00142)	comYH	105629..106582 (+)	954	WP_002987790.1	class I SAM-dependent methyltransferase	Machinery gene
DQL04_RS00705 (NCTC12062_00143)	-	106641..107837 (+)	1197	WP_011284424.1	acetate kinase	-
DQL04_RS00710	-	108024..108332 (+)	309	WP_011284425.1	hypothetical protein	-
DQL04_RS00715 (NCTC12062_00144)	proC	108415..109185 (-)	771	WP_032464773.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16573.20 Da
• Isoelectric Point: 10.3584

>NTDB_id=1137402 DQL04_RS00690 WP_002986542.1 104787..105221(+) (comYF) [Streptococcus pyogenes strain NCTC12062]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1137402 DQL04_RS00690 WP_002986542.1 104787..105221(+) (comYF) [Streptococcus pyogenes strain NCTC12062]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAGACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396