Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DQL82_RS08130 Genome accession   NZ_LS483319
Coordinates   1533837..1534334 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NCTC13140     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1528837..1539334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL82_RS08090 (NCTC13140_01508) - 1528881..1529867 (+) 987 WP_000161314.1 ROK family glucokinase -
  DQL82_RS08095 (NCTC13140_01509) - 1529867..1530196 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DQL82_RS08100 (NCTC13140_01510) - 1530193..1530816 (+) 624 WP_111759442.1 MBL fold metallo-hydrolase -
  DQL82_RS08105 (NCTC13140_01511) comGA 1530868..1531842 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL82_RS08110 (NCTC13140_01512) comGB 1531814..1532884 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL82_RS08115 (NCTC13140_01513) comGC 1532898..1533209 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL82_RS08120 (NCTC13140_01514) comGD 1533187..1533633 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  DQL82_RS08125 (NCTC13140_01515) comGE 1533620..1533919 (+) 300 WP_172449598.1 competence protein ComGE Machinery gene
  DQL82_RS08130 (NCTC13140_01516) comGF 1533837..1534334 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL82_RS08135 - 1534431..1534577 (+) 147 WP_001789879.1 hypothetical protein -
  DQL82_RS08140 - 1534567..1535091 (+) 525 WP_001015117.1 shikimate kinase -
  DQL82_RS08145 (NCTC13140_01518) gcvT 1535250..1536341 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DQL82_RS08150 (NCTC13140_01519) gcvPA 1536361..1537707 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DQL82_RS08155 (NCTC13140_01520) gcvPB 1537700..1539172 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=1136264 DQL82_RS08130 WP_001789864.1 1533837..1534334(+) (comGF) [Staphylococcus aureus strain NCTC13140]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1136264 DQL82_RS08130 WP_001789864.1 1533837..1534334(+) (comGF) [Staphylococcus aureus strain NCTC13140]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment