Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DQL75_RS08200 Genome accession   NZ_LS483317
Coordinates   1625617..1626591 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain NCTC5663     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1620617..1631591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL75_RS08160 (NCTC5663_01552) gcvT 1621118..1622209 (-) 1092 WP_058343671.1 glycine cleavage system aminomethyltransferase GcvT -
  DQL75_RS08165 - 1622368..1622892 (-) 525 WP_029549655.1 shikimate kinase -
  DQL75_RS08170 - 1622882..1623028 (-) 147 WP_001789879.1 hypothetical protein -
  DQL75_RS08175 (NCTC5663_01554) comGF 1623125..1623622 (-) 498 WP_029693246.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL75_RS08180 (NCTC5663_01555) comGE 1623540..1623839 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DQL75_RS08185 (NCTC5663_01556) comGD 1623826..1624272 (-) 447 WP_111762333.1 competence type IV pilus minor pilin ComGD Machinery gene
  DQL75_RS08190 (NCTC5663_01557) comGC 1624250..1624561 (-) 312 WP_111762334.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL75_RS08195 (NCTC5663_01558) comGB 1624575..1625645 (-) 1071 WP_000776405.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL75_RS08200 (NCTC5663_01559) comGA 1625617..1626591 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL75_RS08205 (NCTC5663_01560) - 1626643..1627266 (-) 624 WP_111762335.1 MBL fold metallo-hydrolase -
  DQL75_RS08210 (NCTC5663_01561) - 1627263..1627592 (-) 330 WP_111762336.1 MTH1187 family thiamine-binding protein -
  DQL75_RS08215 (NCTC5663_01562) - 1627592..1628578 (-) 987 WP_000161315.1 ROK family glucokinase -
  DQL75_RS08220 - 1628575..1628778 (-) 204 WP_000087561.1 YqgQ family protein -
  DQL75_RS08225 (NCTC5663_01563) - 1628759..1630222 (-) 1464 WP_001017867.1 rhomboid family protein -
  DQL75_RS08230 (NCTC5663_01564) - 1630234..1630773 (-) 540 WP_111762337.1 5-formyltetrahydrofolate cyclo-ligase -
  DQL75_RS08235 (NCTC5663_01565) rpmG 1630982..1631131 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=1136157 DQL75_RS08200 WP_000697220.1 1625617..1626591(-) (comGA) [Staphylococcus aureus strain NCTC5663]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1136157 DQL75_RS08200 WP_000697220.1 1625617..1626591(-) (comGA) [Staphylococcus aureus strain NCTC5663]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAATTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGGATATCAACTTTACCATTGTCACTTGGTCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGGGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment