Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   EO53_RS00950 Genome accession   NZ_CP009685
Coordinates   213406..214038 (-) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli str. K-12 substr. MG1655 strain K-12     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 208406..219038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO53_RS00925 (EO53_00925) yhfG 208538..208705 (+) 168 WP_000736862.1 YhfG family protein -
  EO53_RS00930 (EO53_00930) fic 208695..209297 (+) 603 WP_001280641.1 putative adenosine monophosphate-protein transferase Fic -
  EO53_RS00935 (EO53_00935) pabA 209329..209892 (+) 564 WP_000601847.1 aminodeoxychorismate synthase component 2 -
  EO53_RS00940 (EO53_00940) argD 209978..211198 (+) 1221 WP_000963792.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  EO53_RS00945 (EO53_00945) yhfK 211265..213355 (-) 2091 WP_001295162.1 YccS/YhfK family putative transporter -
  EO53_RS00950 (EO53_00950) crp 213406..214038 (-) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  EO53_RS00960 (EO53_00960) yhfA 214340..214744 (+) 405 WP_001148908.1 OsmC family protein -
  EO53_RS00965 (EO53_00965) prkB 214799..215668 (-) 870 WP_001274680.1 phosphoribulokinase -
  EO53_RS00970 (EO53_00970) yheU 215722..215940 (-) 219 WP_000907085.1 YheU family protein -
  EO53_RS00975 (EO53_00975) yheT 215934..216956 (-) 1023 WP_000057356.1 hydrolase -
  EO53_RS00980 (EO53_00980) yheS 216956..218869 (-) 1914 WP_000634798.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=113273 EO53_RS00950 WP_000242755.1 213406..214038(-) (crp) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=113273 EO53_RS00950 WP_000242755.1 213406..214038(-) (crp) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467