Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   H1W86_RS00820 Genome accession   NZ_LR822042
Coordinates   139763..141730 (+) Length   655 a.a.
NCBI ID   WP_014607872.1    Uniprot ID   Q8VTU5
Organism   Streptococcus thermophilus isolate STH_CIRM_1358     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 141768..146021 139763..141730 flank 38


Gene organization within MGE regions


Location: 139763..146021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W86_RS00820 (STHERMO_0134) amiA 139763..141730 (+) 1968 WP_014607872.1 peptide ABC transporter substrate-binding protein Regulator
  H1W86_RS00825 (STHERMO_0135) - 141775..142589 (-) 815 Protein_112 ISL3 family transposase -
  H1W86_RS00830 (STHERMO_0136) - 142882..143103 (+) 222 WP_002948904.1 DUF4649 family protein -
  H1W86_RS00835 - 143188..143510 (-) 323 Protein_114 transposase -
  H1W86_RS00840 (STHERMO_0138) - 143699..144025 (+) 327 WP_014607875.1 M20/M25/M40 family metallo-hydrolase -
  H1W86_RS00845 (STHERMO_0140) - 144107..145297 (+) 1191 Protein_116 class I SAM-dependent methyltransferase -
  H1W86_RS00850 (STHERMO_0141) - 145557..146021 (-) 465 Protein_117 transposase family protein -

Sequence

Protein


Download         Length: 655 a.a.        Molecular weight: 71730.84 Da        Isoelectric Point: 5.0409

>NTDB_id=1132096 H1W86_RS00820 WP_014607872.1 139763..141730(+) (amiA) [Streptococcus thermophilus isolate STH_CIRM_1358]
MKLKKILGITGVALLSVGMLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKAVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQEGLYNAEKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMNN
VTSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKIFNGYDDPNNAAAAQVGLKDYDALLDSAASETTDIN
ARYDRYAQAQAWLEDSSLIIPLTVSNGAAPVISRLTPFTGASIQVGDKGSSYVKYVKSQEKVVTKKEYEQSREKWLKERK
ASNEKAQKDLEKHVK

Nucleotide


Download         Length: 1968 bp        

>NTDB_id=1132096 H1W86_RS00820 WP_014607872.1 139763..141730(+) (amiA) [Streptococcus thermophilus isolate STH_CIRM_1358]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTTGGTATGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGCTGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTGACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCAGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATAGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGCGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGAGGGTCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGACATGAATAAT
GTGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGACTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGATTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAGGATTATGATGCCTTGCTTGATTCAGCAGCTTCAGAAACAACAGACATCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTATCATTCCATTGACTGTTAGTAACGG
TGCAGCACCAGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACGTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAGGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VTU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

88.872

100

0.89
  amiA3 Streptococcus thermophilus LMG 18311

86.454

100

0.867
  amiA3 Streptococcus thermophilus LMD-9

86.454

100

0.867

Multiple sequence alignment