Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   GPW48_RS00770 Genome accession   NZ_LR738720
Coordinates   117557..117991 (+) Length   144 a.a.
NCBI ID   WP_011922561.1    Uniprot ID   -
Organism   Streptococcus suis isolate GD-0001     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112557..122991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW48_RS00735 - 113306..113671 (+) 366 WP_002936568.1 DUF1033 family protein -
  GPW48_RS00740 - 113706..114649 (-) 944 Protein_109 S66 family peptidase -
  GPW48_RS00745 comYA 114735..115685 (+) 951 WP_011921718.1 competence type IV pilus ATPase ComGA Machinery gene
  GPW48_RS00750 comYB 115597..116634 (+) 1038 WP_255304289.1 competence type IV pilus assembly protein ComGB Machinery gene
  GPW48_RS00755 comYC 116636..116917 (+) 282 WP_011921720.1 competence type IV pilus major pilin ComGC Machinery gene
  GPW48_RS00760 comGD 116898..117305 (+) 408 WP_011921721.1 competence type IV pilus minor pilin ComGD -
  GPW48_RS00765 comYE 117277..117570 (+) 294 WP_011921722.1 competence type IV pilus minor pilin ComGE Machinery gene
  GPW48_RS00770 comGF/cglF 117557..117991 (+) 435 WP_011922561.1 competence type IV pilus minor pilin ComGF Machinery gene
  GPW48_RS00775 comGG 117969..118379 (+) 411 WP_011921724.1 competence type IV pilus minor pilin ComGG -
  GPW48_RS00780 comYH 118436..119389 (+) 954 WP_024406069.1 class I SAM-dependent methyltransferase Machinery gene
  GPW48_RS00785 - 119439..120626 (+) 1188 WP_012775473.1 acetate kinase -
  GPW48_RS00790 - 120940..121491 (+) 552 WP_012775474.1 folate family ECF transporter S component -
  GPW48_RS00795 - 121547..122803 (+) 1257 WP_012775475.1 bifunctional folylpolyglutamate synthase/dihydrofolate synthase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16491.85 Da        Isoelectric Point: 5.9234

>NTDB_id=1129380 GPW48_RS00770 WP_011922561.1 117557..117991(+) (comGF/cglF) [Streptococcus suis isolate GD-0001]
MLKSKIPAFTLLECLVVLVILSGSLLIFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSWSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1129380 GPW48_RS00770 WP_011922561.1 117557..117991(+) (comGF/cglF) [Streptococcus suis isolate GD-0001]
TTGTTAAAAAGTAAAATTCCTGCTTTTACACTCTTGGAATGTTTGGTGGTTCTAGTCATTCTGTCAGGTAGCCTCTTGAT
TTTTGAAGGCTTATCCAAATTACTAGTCCAGGAAGCGCATTATCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGCCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGTGAA
CAGGCTTTGGCCTTTGGCAAGTCATGGTCGGATGATTTTCGGAAAACAAATGATAGAGGCCAGGGTTACCAACCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GAACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae Rx1

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae D39

52.482

97.917

0.514
  comGF/cglF Streptococcus pneumoniae R6

52.482

97.917

0.514
  comGF/cglF Streptococcus mitis NCTC 12261

52.985

93.056

0.493
  comYF Streptococcus mutans UA140

48.611

100

0.486
  comYF Streptococcus mutans UA159

47.917

100

0.479
  comGF/cglF Streptococcus mitis SK321

50

93.056

0.465
  comGF Lactococcus lactis subsp. cremoris KW2

45.39

97.917

0.444

Multiple sequence alignment